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Literature summary for 2.4.2.17 extracted from

  • Fisher, G.; Corbella, M.; Alphey, M.S.; Nicholson, J.; Read, B.J.; Kamerlin, S.C.L.; da Silva, R.G.
    Allosteric rescue of catalytically impaired ATP phosphoribosyltransferase variants links protein dynamics to active-site electrostatic preorganisation (2022), Nat. Commun., 13, 7607.
    View publication on PubMed
Search for more literature for 2.4.2.17

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme R56A,vapor diffusion method Psychrobacter arcticus

Protein Variants

Protein Variants Comment Organism
C115A the mutant shows reduced activity compared to the wild type enzyme Psychrobacter arcticus
C115S the mutant shows 117fold reduced activity compared to the wild type enzyme Psychrobacter arcticus
D179A the mutant shows reduced activity compared to the wild type enzyme Psychrobacter arcticus
D179N the mutant shows reduced activity compared to the wild type enzyme Psychrobacter arcticus
R32A the mutant shows 25fold reduced activity compared to the wild type enzyme Psychrobacter arcticus
R56A the mutant shows 42fold reduced activity compared to the wild type enzyme. The active site HisGS mutant shows impaired reaction chemistry which can be allosterically restored by subunit HisZ. HisZ modulates Arg32 dynamics so that it can partially compensate for the absence of Arg56 Psychrobacter arcticus
R56A/K57A the mutant shows 254fold reduced activity compared to the wild type enzyme Psychrobacter arcticus

Inhibitors

Inhibitors Comment Organism Structure
L-histidine
-
 Psychrobacter arcticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.37
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A, at pH 8.5 and 20°C Psychrobacter arcticus
0.4
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A/K57A, at pH 8.5 and 20°C Psychrobacter arcticus
0.41
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R32A, at pH 8.5 and 20°C Psychrobacter arcticus
0.44
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type enzyme, at pH 8.5 and 20°C Psychrobacter arcticus
0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme C115S, at pH 8.5 and 20°C Psychrobacter arcticus
0.76
-
 ATP wild type enzyme, at pH 8.5 and 20°C Psychrobacter arcticus
0.9
-
 ATP mutant enzyme R56A, at pH 8.5 and 20°C Psychrobacter arcticus
1.5
-
 ATP mutant enzyme R32A, at pH 8.5 and 20°C Psychrobacter arcticus
1.5
-
 ATP mutant enzyme R56A/K57A, at pH 8.5 and 20°C Psychrobacter arcticus
2.8
-
 ATP mutant enzyme C115S, at pH 8.5 and 20°C Psychrobacter arcticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on Psychrobacter arcticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus DSM 17307
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Psychrobacter arcticus Q4FQF7 AND Q4FTX3 subunits HisG and HisZ
-
Psychrobacter arcticus DSM 17307 Q4FQF7 AND Q4FTX3 subunits HisG and HisZ
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus DSM 17307 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus DSM 17307 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Synonyms

Synonyms Comment Organism
ATPPRT
-
 Psychrobacter arcticus
HisGs catalytic subunit Psychrobacter arcticus
HisZ regulatory subunit Psychrobacter arcticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.12
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A/K57A, at pH 8.5 and 20°C Psychrobacter arcticus
0.12
-
 ATP mutant enzyme R56A/K57A, at pH 8.5 and 20°C Psychrobacter arcticus
0.35
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R56A, at pH 8.5 and 20°C Psychrobacter arcticus
0.35
-
 ATP mutant enzyme R56A, at pH 8.5 and 20°C Psychrobacter arcticus
0.48
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme R32A, at pH 8.5 and 20°C Psychrobacter arcticus
0.48
-
 ATP mutant enzyme R32A, at pH 8.5 and 20°C Psychrobacter arcticus
0.93
-
 5-phospho-alpha-D-ribose 1-diphosphate mutant enzyme C115S, at pH 8.5 and 20°C Psychrobacter arcticus
0.93
-
 ATP mutant enzyme C115S, at pH 8.5 and 20°C Psychrobacter arcticus
1.72
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type enzyme, at pH 8.5 and 20°C Psychrobacter arcticus
1.72
-
 ATP wild type enzyme, at pH 8.5 and 20°C Psychrobacter arcticus

General Information

General Information Comment Organism
metabolism the enzyme catalyzes the first step of histidine biosynthesis and is controlled via a complex allosteric mechanism where the regulatory protein HisZ enhances catalysis by the catalytic protein HisGS while mediating allosteric inhibition by histidine Psychrobacter arcticus