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Literature summary for 2.4.2.17 extracted from

  • Stroek, R.; Ge, Y.; Talbot, P.D.; Glok, M.K.; Bernas, K.E.; Thomson, C.M.; Gould, E.R.; Alphey, M.S.; Liu, H.; Florence, G.J.; Naismith, J.H.; da Silva, R.G.
    Kinetics and structure of a cold-adapted hetero-octameric ATP phosphoribosyltransferase (2017), Biochemistry, 56, 793-803.
    View publication on PubMed
Search for more literature for 2.4.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C43(DE3) cells Psychrobacter arcticus

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 11% (w/v) PEG 3350, 0.1 M bicine (pH 8.5), 0.15 M SrCl2, 0.15 M KBr, and 2% (v/v) 1,6-hexanediol Psychrobacter arcticus

Inhibitors

Inhibitors Comment Organism Structure
AMP
-
 Psychrobacter arcticus
L-histidine
-
 Psychrobacter arcticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
0.6
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
0.7
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
0.7
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus
0.8
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
1.2
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
1.7
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
1.8
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
2
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
2.1
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ dependent on, 10 mM used in assay conditions Psychrobacter arcticus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Psychrobacter arcticus DSM 17307
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Psychrobacter arcticus
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Psychrobacter arcticus DSM 17307
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Psychrobacter arcticus Q4FQF7
-
-
Psychrobacter arcticus DSM 17307 Q4FQF7
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Psychrobacter arcticus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Psychrobacter arcticus DSM 17307 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Psychrobacter arcticus DSM 17307 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

Subunits Comment Organism
heterooctamer 4 * 40000 + 4 * 23000, subunits HisZ and HisGS, SDS-PAGE Psychrobacter arcticus

Synonyms

Synonyms Comment Organism
adenosine 5'-triphosphate phosphoribosyltransferase
-
 Psychrobacter arcticus
ATPPRT
-
 Psychrobacter arcticus
HisG
-
 Psychrobacter arcticus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
38.9
-
 melting temperature for subunit HisZ Psychrobacter arcticus
42.9
-
 melting temperature for subunit HisGS Psychrobacter arcticus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.45
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus
0.45
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
0.64
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
0.64
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
0.94
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
0.94
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
0.94
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
1.16
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
1.16
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.214
-
 ATP at pH 8.5 and 10°C Psychrobacter arcticus
0.32
-
 ATP at pH 8.5 and 15°C Psychrobacter arcticus
0.422
-
 ATP at pH 8.5 and 20°C Psychrobacter arcticus
0.682
-
 ATP at pH 8.5 and 25°C Psychrobacter arcticus
0.75
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 10°C Psychrobacter arcticus
0.783
-
 ATP at pH 8.5 and 30°C Psychrobacter arcticus
0.915
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 15°C Psychrobacter arcticus
1.175
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 30°C Psychrobacter arcticus
1.266
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 20°C Psychrobacter arcticus
1.657
-
 5-phospho-alpha-D-ribose 1-diphosphate at pH 8.5 and 25°C Psychrobacter arcticus