Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C43(DE3) cells | Psychrobacter arcticus |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 11% (w/v) PEG 3350, 0.1 M bicine (pH 8.5), 0.15 M SrCl2, 0.15 M KBr, and 2% (v/v) 1,6-hexanediol | Psychrobacter arcticus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Psychrobacter arcticus | |
L-histidine | - |
Psychrobacter arcticus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 10°C | Psychrobacter arcticus | |
0.6 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 20°C | Psychrobacter arcticus | |
0.7 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Psychrobacter arcticus | |
0.7 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 15°C | Psychrobacter arcticus | |
0.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 30°C | Psychrobacter arcticus | |
1.2 | - |
ATP | at pH 8.5 and 30°C | Psychrobacter arcticus | |
1.7 | - |
ATP | at pH 8.5 and 25°C | Psychrobacter arcticus | |
1.8 | - |
ATP | at pH 8.5 and 20°C | Psychrobacter arcticus | |
2 | - |
ATP | at pH 8.5 and 15°C | Psychrobacter arcticus | |
2.1 | - |
ATP | at pH 8.5 and 10°C | Psychrobacter arcticus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, 10 mM used in assay conditions | Psychrobacter arcticus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Psychrobacter arcticus | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Psychrobacter arcticus DSM 17307 | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Psychrobacter arcticus | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Psychrobacter arcticus DSM 17307 | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Psychrobacter arcticus | Q4FQF7 | - |
- |
Psychrobacter arcticus DSM 17307 | Q4FQF7 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Psychrobacter arcticus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Psychrobacter arcticus | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Psychrobacter arcticus DSM 17307 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Psychrobacter arcticus | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Psychrobacter arcticus DSM 17307 | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
heterooctamer | 4 * 40000 + 4 * 23000, subunits HisZ and HisGS, SDS-PAGE | Psychrobacter arcticus |
Synonyms | Comment | Organism |
---|---|---|
adenosine 5'-triphosphate phosphoribosyltransferase | - |
Psychrobacter arcticus |
ATPPRT | - |
Psychrobacter arcticus |
HisG | - |
Psychrobacter arcticus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
38.9 | - |
melting temperature for subunit HisZ | Psychrobacter arcticus |
42.9 | - |
melting temperature for subunit HisGS | Psychrobacter arcticus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
ATP | at pH 8.5 and 10°C | Psychrobacter arcticus | |
0.45 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 10°C | Psychrobacter arcticus | |
0.64 | - |
ATP | at pH 8.5 and 15°C | Psychrobacter arcticus | |
0.64 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 15°C | Psychrobacter arcticus | |
0.94 | - |
ATP | at pH 8.5 and 30°C | Psychrobacter arcticus | |
0.94 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 30°C | Psychrobacter arcticus | |
0.94 | - |
ATP | at pH 8.5 and 20°C | Psychrobacter arcticus | |
0.94 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 20°C | Psychrobacter arcticus | |
1.16 | - |
ATP | at pH 8.5 and 25°C | Psychrobacter arcticus | |
1.16 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Psychrobacter arcticus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.214 | - |
ATP | at pH 8.5 and 10°C | Psychrobacter arcticus | |
0.32 | - |
ATP | at pH 8.5 and 15°C | Psychrobacter arcticus | |
0.422 | - |
ATP | at pH 8.5 and 20°C | Psychrobacter arcticus | |
0.682 | - |
ATP | at pH 8.5 and 25°C | Psychrobacter arcticus | |
0.75 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 10°C | Psychrobacter arcticus | |
0.783 | - |
ATP | at pH 8.5 and 30°C | Psychrobacter arcticus | |
0.915 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 15°C | Psychrobacter arcticus | |
1.175 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 30°C | Psychrobacter arcticus | |
1.266 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 20°C | Psychrobacter arcticus | |
1.657 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Psychrobacter arcticus |