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Literature summary for 2.4.2.17 extracted from

  • Mittelstaedt, G.; Jiao, W.; Livingstone, E.K.; Moggre, G.J.; Nazmi, A.R.; Parker, E.J.
    A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase (2018), Biochem. J., 475, 247-260.
    View publication on PubMed
Search for more literature for 2.4.2.17

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment) Organism
truncated long-form enzyme devoid of its regulatory domain as apoenzyme and in complex with ATP, 5-phospho-alpha-D-ribose 1-diphosphate or 1-(5-phospho-beta-D-ribosyl)-ATP, hanging drop vapor diffusion method, using 1 M sodium acetate, 10 mM ZnCl2, and 7-10% (w/v) PEG 6000 (pH 5.0) Campylobacter jejuni

Inhibitors

Inhibitors Comment Organism Structure
1-(5-phospho-beta-D-ribosyl)-ATP product inhibition Campylobacter jejuni
AMP competitive inhibition Campylobacter jejuni
L-histidine allosterical inhibition Campylobacter jejuni

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni
0.028
-
 5-phospho-alpha-D-ribose 1-diphosphate truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni
0.097
-
 ATP wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni
0.117
-
 ATP truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM used in assay conditions Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25308
-
 truncated long-form enzyme devoid of its regulatory domain, calculated from amino acid sequence Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate Campylobacter jejuni RM 1221
-
 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Campylobacter jejuni RM 1221
-
 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Organism

Organism UniProt Comment Textmining
Campylobacter jejuni Q5HSJ4
-
-
Campylobacter jejuni RM 1221 Q5HSJ4
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Talon resin column chromatography, GST-affinity column chromatography and Superdex S200 gel filtration Campylobacter jejuni

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 Campylobacter jejuni RM 1221 ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Campylobacter jejuni RM 1221 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate
-
 r

Subunits

Subunits Comment Organism
homohexamer long enzyme form Campylobacter jejuni

Synonyms

Synonyms Comment Organism
adenosine triphosphate phosphoribosyltransferase
-
 Campylobacter jejuni
ATP-phosphoribosyltransferase
-
 Campylobacter jejuni
ATP-PRT
-
 Campylobacter jejuni

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52.5
-
 melting temperature of the truncated long-form enzyme devoid of its regulatory domain Campylobacter jejuni
60.7
-
 melting temperature of the wild type long-form enzyme Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.024
-
 ATP truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni
0.024
-
 5-phospho-alpha-D-ribose 1-diphosphate truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni
1.8
-
 ATP wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni
1.8
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0068
-
 truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni 1-(5-phospho-beta-D-ribosyl)-ATP
0.04
-
 wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni 1-(5-phospho-beta-D-ribosyl)-ATP
0.044
-
 wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni L-histidine
0.42
-
 truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni AMP
2.1
-
 wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni AMP

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.21
-
 ATP truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni
0.86
-
 5-phospho-alpha-D-ribose 1-diphosphate truncated long-form enzyme devoid of its regulatory domain, at pH 8.5 and 25°C Campylobacter jejuni
19
-
 ATP wild type long-form enzyme, at pH 8.5 and 25°C Campylobacter jejuni
120
-
 5-phospho-alpha-D-ribose 1-diphosphate wild type long-form enzyme , at pH 8.5 and 25°C Campylobacter jejuni