Literature summary for 2.4.2.17 extracted from

Mittelstaedt, G.; Moggre, G.J.; Panjikar, S.; Nazmi, A.R.; Parker, E.J.
Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer which is allosterically controlled by the twisting of a regulatory tail (2016), Protein Sci., 25, 1492-1506.

Search for more literature for 2.4.2.17

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Campylobacter jejuni

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with ATP, L-histidine, or L-histidine/AMP, hanging drop vapor diffusion method, using 0.1M sodium acetate, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 5.5 (ATP), or 0.1 M BTP, 0.2 M KSCN, 13-14% (w/v) PEG 3350, pH 6.5 (His), or 0.1 M Tris, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 7.5 (L-His/AMP)	Campylobacter jejuni

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP	competitive inhibition	Campylobacter jejuni
L-histidine	allosterical inhibition	Campylobacter jejuni

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.015	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 8.5 and 25°C	Campylobacter jejuni
0.097	-	ATP	at pH 8.5 and 25°C	Campylobacter jejuni

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essential	Campylobacter jejuni

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
184000	-	gel filtration	Campylobacter jejuni
188000	-	SEC-coupled static light scattering	Campylobacter jejuni
202000	-	calculated from amino acid sequence	Campylobacter jejuni

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	Campylobacter jejuni	-	ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	Campylobacter jejuni NCTC 11168	-	ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	Campylobacter jejuni	-	1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	Campylobacter jejuni NCTC 11168	-	1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Campylobacter jejuni	Q9PM78	-	-
Campylobacter jejuni NCTC 11168	Q9PM78	-	-

Purification (Commentary)

Purification (Comment)	Organism
HiTrap Talon column chromatography and Superdex 200 gel filtration	Campylobacter jejuni

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	Campylobacter jejuni	ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	Campylobacter jejuni NCTC 11168	ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	Campylobacter jejuni	1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	Campylobacter jejuni NCTC 11168	1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate	-	r

Subunits

Subunits	Comment	Organism
hexamer	-	Campylobacter jejuni

Synonyms

Synonyms	Comment	Organism
adenosine triphosphate phosphoribosyltransferase	-	Campylobacter jejuni
ATP-PRT	-	Campylobacter jejuni
HisGL	-	Campylobacter jejuni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.8	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 8.5 and 25°C	Campylobacter jejuni

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.04	-	L-histidine	with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C	Campylobacter jejuni
0.044	-	L-histidine	with respect to ATP, at pH 8.5 and 25°C	Campylobacter jejuni
0.356	-	AMP	with respect to ATP, at pH 8.5 and 25°C	Campylobacter jejuni
0.382	-	AMP	with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C	Campylobacter jejuni

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
19	-	ATP	at pH 8.5 and 25°C	Campylobacter jejuni
120	-	5-phospho-alpha-D-ribose 1-diphosphate	at pH 8.5 and 25°C	Campylobacter jejuni

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Release 2023.1 (January 2023)