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Literature summary for 2.4.2.17 extracted from
- Campylobacter jejuni adenosine triphosphate phosphoribosyltransferase is an active hexamer which is allosterically controlled by the twisting of a regulatory tail (2016), Protein Sci., 25, 1492-1506.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Campylobacter jejuni |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| in complex with ATP, L-histidine, or L-histidine/AMP, hanging drop vapor diffusion method, using 0.1M sodium acetate, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 5.5 (ATP), or 0.1 M BTP, 0.2 M KSCN, 13-14% (w/v) PEG 3350, pH 6.5 (His), or 0.1 M Tris, 0.1 M MgCl2, 13-15% (w/v) PEG 4000, pH 7.5 (L-His/AMP) | Campylobacter jejuni |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | competitive inhibition | Campylobacter jejuni |  |
| L-histidine | allosterical inhibition | Campylobacter jejuni | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.015 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni | |
| 0.097 | - |
ATP | at pH 8.5 and 25°C | Campylobacter jejuni | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | essential | Campylobacter jejuni | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 184000 | - |
gel filtration | Campylobacter jejuni |
| 188000 | - |
SEC-coupled static light scattering | Campylobacter jejuni |
| 202000 | - |
calculated from amino acid sequence | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | Campylobacter jejuni NCTC 11168 | - |
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Campylobacter jejuni NCTC 11168 | - |
1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Campylobacter jejuni | Q9PM78 | - |
- |
| Campylobacter jejuni NCTC 11168 | Q9PM78 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap Talon column chromatography and Superdex 200 gel filtration | Campylobacter jejuni |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
Campylobacter jejuni NCTC 11168 | ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Campylobacter jejuni NCTC 11168 | 1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| hexamer | - |
Campylobacter jejuni |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| adenosine triphosphate phosphoribosyltransferase | - |
Campylobacter jejuni |
| ATP-PRT | - |
Campylobacter jejuni |
| HisGL | - |
Campylobacter jejuni |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.8 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
L-histidine | with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C | Campylobacter jejuni | |
| 0.044 | - |
L-histidine | with respect to ATP, at pH 8.5 and 25°C | Campylobacter jejuni | |
| 0.356 | - |
AMP | with respect to ATP, at pH 8.5 and 25°C | Campylobacter jejuni | |
| 0.382 | - |
AMP | with respect to 5-phospho-alpha-D-ribose 1-diphosphate, at pH 8.5 and 25°C | Campylobacter jejuni | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19 | - |
ATP | at pH 8.5 and 25°C | Campylobacter jejuni | |
| 120 | - |
5-phospho-alpha-D-ribose 1-diphosphate | at pH 8.5 and 25°C | Campylobacter jejuni | |
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