Cloned (Comment) | Organism |
---|---|
His-tagged protein expressed in Escherichia coli BL21(DE3) | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
A270P | conserved residue | Corynebacterium glutamicum |
L231F/T235A | conserved residues | Corynebacterium glutamicum |
N215K | conserved residue | Corynebacterium glutamicum |
N215K/L231F/T235A | conserved residues, 37fold increase in Ki-value of histidine | Corynebacterium glutamicum |
N215K/L231F/T235A/A270P | conserved residues | Corynebacterium glutamicum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | competitive to ATP | Corynebacterium glutamicum | |
Ag+ | - |
Corynebacterium glutamicum | |
AMP | competitive to ATP | Corynebacterium glutamicum | |
Ca2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
Cu2+ | - |
Corynebacterium glutamicum | |
EDTA | moderate inhibitory effect | Corynebacterium glutamicum | |
Fe2+ | - |
Corynebacterium glutamicum | |
Fe3+ | - |
Corynebacterium glutamicum | |
Hg2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
L-histidine | noncompetitive, alkaline pH decreases the inhibitory effect | Corynebacterium glutamicum | |
Mn2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
Zn2+ | moderate inhibitory effect | Corynebacterium glutamicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
5-phospho-alpha-D-ribose 1-diphosphate | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.09 | - |
5-phospho-alpha-D-ribose 1-diphosphate | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.22 | - |
ATP | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.22 | - |
ATP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.23 | - |
ATP | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.24 | - |
ATP | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.35 | - |
ATP | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Corynebacterium glutamicum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32191 | - |
2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
32191 | - |
6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
63000 | - |
homodimer, gel filtration | Corynebacterium glutamicum |
192000 | - |
homohexamer, gel filtration | Corynebacterium glutamicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | Q9Z472 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity chromatography, gel filtration | Corynebacterium glutamicum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.39 | - |
N215K/L231F/T235A/A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
1.96 | - |
A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
2.01 | - |
N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
2.04 | - |
L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
2.1 | - |
N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
2.19 | - |
wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Corynebacterium glutamicum | 1-(5-phospho-alpha-D-ribosyl)-ATP + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
homohexamer | 6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
Synonyms | Comment | Organism |
---|---|---|
ATP phosphoribosyltransferase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Corynebacterium glutamicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | - |
Corynebacterium glutamicum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.29 | - |
5-phospho-alpha-D-ribose 1-diphosphate | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
1.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
1.75 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
1.91 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
2.22 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Corynebacterium glutamicum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.5 | 13 | >40% relative activity, rapid loss of activity below pH 7.5 | Corynebacterium glutamicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-histidine | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.24 | - |
L-histidine | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.28 | - |
L-histidine | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.52 | - |
L-histidine | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
0.88 | - |
ADP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
1.29 | - |
AMP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
4.15 | - |
L-histidine | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
General Information | Comment | Organism |
---|---|---|
metabolism | essential for histidine biosynthesis | Corynebacterium glutamicum |