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Literature summary for 2.4.2.17 extracted from
- Genetic and biochemical characterization of Corynebacterium glutamicum ATP phosphoribosyltransferase and its three mutants resistant to feedback inhibition by histidine (2012), Biochimie, 94, 829-838.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| His-tagged protein expressed in Escherichia coli BL21(DE3) | Corynebacterium glutamicum |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| A270P | conserved residue | Corynebacterium glutamicum |
| L231F/T235A | conserved residues | Corynebacterium glutamicum |
| N215K | conserved residue | Corynebacterium glutamicum |
| N215K/L231F/T235A | conserved residues, 37fold increase in Ki-value of histidine | Corynebacterium glutamicum |
| N215K/L231F/T235A/A270P | conserved residues | Corynebacterium glutamicum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | competitive to ATP | Corynebacterium glutamicum |  |
| Ag+ | - |
Corynebacterium glutamicum | |
| AMP | competitive to ATP | Corynebacterium glutamicum | |
| Ca2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
| Cu2+ | - |
Corynebacterium glutamicum | |
| EDTA | moderate inhibitory effect | Corynebacterium glutamicum | |
| Fe2+ | - |
Corynebacterium glutamicum | |
| Fe3+ | - |
Corynebacterium glutamicum | |
| Hg2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
| L-histidine | noncompetitive, alkaline pH decreases the inhibitory effect | Corynebacterium glutamicum | |
| Mn2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
| Zn2+ | moderate inhibitory effect | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
5-phospho-alpha-D-ribose 1-diphosphate | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.08 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.09 | - |
5-phospho-alpha-D-ribose 1-diphosphate | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.22 | - |
ATP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.22 | - |
ATP | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.23 | - |
ATP | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.24 | - |
ATP | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.35 | - |
ATP | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32191 | - |
2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
| 32191 | - |
6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
| 63000 | - |
homodimer, gel filtration | Corynebacterium glutamicum |
| 192000 | - |
homohexamer, gel filtration | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | Q9Z472 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| immobilized metal ion affinity chromatography, gel filtration | Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.39 | - |
N215K/L231F/T235A/A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
| 1.96 | - |
A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
| 2.01 | - |
N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
| 2.04 | - |
L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
| 2.1 | - |
N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
| 2.19 | - |
wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Corynebacterium glutamicum | 1-(5-phospho-alpha-D-ribosyl)-ATP + diphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
| homohexamer | 6 * 32191, MALDI-TOF MS, gel filtration, theoretical molecular mass 31000 | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP phosphoribosyltransferase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Corynebacterium glutamicum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | - |
Corynebacterium glutamicum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.29 | - |
5-phospho-alpha-D-ribose 1-diphosphate | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.36 | - |
5-phospho-alpha-D-ribose 1-diphosphate | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.75 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.91 | - |
5-phospho-alpha-D-ribose 1-diphosphate | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 2.22 | - |
5-phospho-alpha-D-ribose 1-diphosphate | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 10 | - |
- |
Corynebacterium glutamicum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 13 | >40% relative activity, rapid loss of activity below pH 7.5 | Corynebacterium glutamicum |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.11 | - |
L-histidine | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.24 | - |
L-histidine | A270P mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.28 | - |
L-histidine | N215K mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.52 | - |
L-histidine | L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 0.88 | - |
ADP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 1.29 | - |
AMP | wild type protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
| 4.15 | - |
L-histidine | N215K/L231F/T235A mutant protein, pH 8.5, temperature not specified in the publication | Corynebacterium glutamicum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | essential for histidine biosynthesis | Corynebacterium glutamicum |
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