Literature summary for 2.4.2.17 extracted from

Vega, M.C.; Zou, P.; Fernandez, F.J.; Murphy, G.E.; Sterner, R.; Popov, A.; Wilmanns, M.
Regulation of the hetero-octameric ATP phosphoribosyl transferase complex from Thermotoga maritima by a tRNA synthetase-like subunit (2005), Mol. Microbiol., 55, 675-686.

Cloned(Commentary)

Cloned (Comment)	Organism
separate expression of subunits HisGs and HisZ in Escherichia coli strain BL21(DE3) as wild-type, or in strain B834 as selenomethionine-labeled proteins	Thermotoga maritima

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled subunits HisGs and HisZ separately, in a binary complex with histidine, sitting drop vapour diffusion method, 0.002 ml of 8 mg/ml protein mixed with 0.002 ml reservoir solution containing 22.5% w/v methyl-2,4-pentanediol, 0.2 M phosphate/citrate buffer, pH 4.2, 2 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling	Thermotoga maritima

Crystallization (Comment)

Organism

purified recombinant wild-type and selenomethionine-labeled subunits HisGs and HisZ separately, in a binary complex with histidine, sitting drop vapour diffusion method, 0.002 ml of 8 mg/ml protein mixed with 0.002 ml reservoir solution containing 22.5% w/v methyl-2,4-pentanediol, 0.2 M phosphate/citrate buffer, pH 4.2, 2 days, X-ray diffraction structure determination and analysis at 2.5 A resolution, modeling

Thermotoga maritima

Inhibitors

Inhibitors	Comment	Organism	Structure
histidine	noncompetitive feedback inhibition	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
additional information	-	additional information	steady-state kinetics	Thermotoga maritima

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	dependent on	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
200000	220000	gel filtration	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	Thermotoga maritima	first step in histidine biosynthesis, mechanism of regulation, overview	1-(5-phospho-D-ribosyl)-ATP + diphosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant wild-type and selenomethionine-labeled subunits HisGs and HisZ from Escherichia coli in a multistep procedure	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	first step in histidine biosynthesis, mechanism of regulation, overview	Thermotoga maritima	1-(5-phospho-D-ribosyl)-ATP + diphosphate	-	r
ATP + 5-phospho-alpha-D-ribose 1-diphosphate	-	Thermotoga maritima	1-(5-phospho-D-ribosyl)-ATP + diphosphate	-	r
additional information	the enzyme comprises 4 catalytic subunits HisGs and 4 regulatory subunits HisZ, only the complete hetero-octameric complex is catalytically active, the complex possesses 8 histidine binding sites at the subunit interfaces and histidine as a ligand	Thermotoga maritima	?	-	?

Subunits

Subunits	Comment	Organism
additional information	subunit structures, structure evolution	Thermotoga maritima
octamer	4 * catalytic subunit HisGs + 4 * regulatory subunit HisZ, (alpha,beta)4	Thermotoga maritima

Synonyms

Synonyms	Comment	Organism
ATP phosphoribosyl transferase	-	Thermotoga maritima
ATP phosphoribosyl transferase complex	-	Thermotoga maritima
ATP-PRTase	-	Thermotoga maritima
N-1-(5'-phosphoribosyl)-ATP transferase	-	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
20	-	assay at	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
8	-	assay at	Thermotoga maritima