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Literature summary for 2.4.2.17 extracted from

  • Lohkamp, B.; Coggins, J.R.; Lapthorn, A.J.
    Purification, crystallization and preliminary x-ray crystallographic analysis of ATP-phosphoribosyltransferase from Escherichia coli (2000), Acta Crystallogr. Sect. D, 56, 1488-1491.
    View publication on PubMed
Search for more literature for 2.4.2.17

Crystallization (Commentary)

Crystallization (Comment) Organism
vapour diffusion method, trigonal prisms are obtained using 1.3 M sodium tartrate, 50-200 mM magnesium chloride, 100 mM citrate buffer pH 5.6 and enzyme in the presence of 2 mM AMP, round shaped crystals are obtained with 1.36-1.44 M ammonium sulfate, 0-0.3 M sodium chloride, 100 mM HEPES buffer pH 7.5 and enzyme in the presence of 2 mM AMP Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-histidine feed-back inhibition Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33367
-
 6 * 33367, dimers arranged in a hexamer, in the presence of AMP Escherichia coli
186000
-
 dynamic light scattering in the presence of AMP or histidine Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Escherichia coli first step in histidine biosynthesis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
centrifugation and DEAE-Sephacel anion-exchange column Escherichia coli

Storage Stability

Storage Stability Organism
-20°C, 50 mM Tris-HCl, pH 7.5, 0.4 mM DTT and one protease inhibitor per litre, 50% v/v glycerol, long term storage Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Escherichia coli 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?
ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Escherichia coli diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 33367, dimers arranged in a hexamer, in the presence of AMP Escherichia coli