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Literature summary for 2.4.2.17 extracted from
- Molecular cloning and characterization of ATP-phosphoribosyl transferase from Arabidopsis, a key enzyme in the histidine biosynthetic pathway (2000), Plant Physiol., 122, 907-914.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 1,2,4-Triazole-3-alanine | - |
Arabidopsis thaliana |  |
| L-histidine | feed-back inhibition; feed-back inhibition | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
5-phospho-alpha-D-ribose 1-diphosphate | recombinant At-ATP-PRT1 | Arabidopsis thaliana | |
| 0.51 | - |
ATP | crude cell extracts | Arabidopsis thaliana | |
| 0.57 | - |
5-phospho-alpha-D-ribose 1-diphosphate | crude cell extracts | Arabidopsis thaliana | |
| 0.6 | - |
ATP | recombinant enzyme | Arabidopsis thaliana | |
| 37 | - |
5-phospho-alpha-D-ribose 1-diphosphate | crude cell extracts | Arabidopsis thaliana | |
| 89 | - |
ATP | crude cell extracts | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44600 | - |
calculated mass from amino acid sequence | Arabidopsis thaliana |
| 44800 | - |
calculated mass from amino acid sequence | Arabidopsis thaliana |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | Arabidopsis thaliana | first step in histidine biosynthesis | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
At-ATP-PRT2 | - |
| Arabidopsis thaliana | - |
ecotype Columbia | - |
| Arabidopsis thaliana | Q9S762 | At-ATP-PRT1 | - |
| Arabidopsis thaliana | Q9S762 | ecotype Columbia | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| the recombinant enzymes are produced as fused proteins with a maltose-binding protein, and the purification is made by a two step amylose resin column followed by a digestion with Factor Xa | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Arabidopsis thaliana | 1-(5-phospho-D-ribosyl)-ATP + diphosphate | - |
? | |
| ATP + 5-phospho-alpha-D-ribose 1-diphosphate | first step in histidine biosynthesis | Arabidopsis thaliana | diphosphate + N-1-(5'-phosphoribosyl)-ATP | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
assay at | Arabidopsis thaliana |
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