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Literature summary for 2.4.2.17 extracted from

  • Parsons, S.M.; Koshland, D.E.
    A rapid isolation of phosphoribosyladenosine triphosphate synthetase and comparison to native enzyme (1974), J. Biol. Chem., 249, 4104-4109.
    View publication on PubMed
Search for more literature for 2.4.2.17

General Stability

General Stability Organism
overview, stability of the enzyme at various pH-values, salt concentrations and histidine concentrations Salmonella enterica subsp. enterica serovar Typhimurium
slight stabilization by 10 mM MgCl2 or CaCl2 by 1 mM MnCl2 and by 1 mM histidine at pH-values above 7 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
L-histidine feed-back inhibition Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 6 * 33000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium
216000
-
 ultracentrifugation analysis Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Salmonella enterica subsp. enterica serovar Typhimurium first step in histidine biosynthesis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
first purification by heating the crude extract, ammonium sulfate precipitation and selective histidine-dependent ammonium sulfate precipitation Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 description of assay method Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, HEPES buffer pH 7.5, final ammonium sulfate microcrystalline enzyme in 60% saturated ammonium sulfate, 0.1 M NaCl, 0.01 M Tris, 1.5 mM EDTA, 10 mM dithiothreitol, stable for 1 month, greater than 95% activity retained Salmonella enterica subsp. enterica serovar Typhimurium
storage in liquid nitrogen of quick-frozen enzyme in HEPES buffer pH 7.5, 0.1 M NaCl, 0.01 M Tris, 0.5 mM EDTA, 1 mM dithiothreitol, 1 mM histidine, indefinitely stable, preserves 100% activity. The critical factor for stability seems to be the maintenance of a sulfhydryl-reducing environment, high dithiothreitol concentrations has no adverse effect at either 0°C or 37°C Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?
ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 33000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
 overview: stability at various pH-values Salmonella enterica subsp. enterica serovar Typhimurium