Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.17 extracted from

  • Voll, M.J.; Appella, E.; Martin, R.G.
    Purification and composition studies of phosphoribosyladenosine triphosphate:pyrophosphate phosphoribosyltransferase, the first enzyme of histidine biosynthesis (1967), J. Biol. Chem., 242, 1760-1767.
    View publication on PubMed
Search for more literature for 2.4.2.17

Activating Compound

Activating Compound Comment Organism Structure
additional information other salts like ammonium sulfate or ammonium chloride show the same effect as KCl Salmonella enterica subsp. enterica serovar Typhimurium
potassium chloride stimulates ATP-PRT activity in crude extracts, adding KCl to a final concentration of 0.13 M in reaction mixture increases the reaction rate by about 40% Salmonella enterica subsp. enterica serovar Typhimurium

General Stability

General Stability Organism
NaCl and 2-mercaptoethanol stabilize the very labile enzyme Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
L-histidine feed-back inhibition Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
 6 * 36000, viscometric methods, equilibrium centrifugation with meniscus depletion method after dialysis against 5.0 M guanidine-HCl and 0.143 M 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium
210000 221000 sedimentation, equilibrium centrifugation with meniscus depletion method Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate Salmonella enterica subsp. enterica serovar Typhimurium first step in histidine biosynthesis diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Mn2+ precipitation, DEAE-cellulose and ammonium sulfate precipitation, later on Sephadex G-150 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

Storage Stability Organism
4°C, 0.01 M Tris, 0.10 M NaCl, 0.4 mM histidine, 2.8 mM 2-mercaptoethanol, 0.5 mM EDTA, pH 7.5, 50% loss of activity after several days Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium 1-(5-phospho-D-ribosyl)-ATP + diphosphate
-
 ?
ATP + 5-phospho-alpha-D-ribose 1-diphosphate first step in histidine biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium diphosphate + N-1-(5'-phosphoribosyl)-ATP
-
 ?

Subunits

Subunits Comment Organism
hexamer 6 * 36000, viscometric methods, equilibrium centrifugation with meniscus depletion method after dialysis against 5.0 M guanidine-HCl and 0.143 M 2-mercaptoethanol Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium