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Literature summary for 2.4.2.15 extracted from
- The phosphorolysis of nucleosides by rabbit bone marrow (1961), J. Biol. Chem., 236, 3043-3046.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | required for optimal activity | Oryctolagus cuniculus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| PCMB | 0.2 mM, complete inhibition | Oryctolagus cuniculus | |
| Tris buffer | 0.1 M, pH 7.5, 22% inhibition | Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.21 | - |
deoxyguanosine | - |
Oryctolagus cuniculus | |
| 0.216 | - |
guanosine | - |
Oryctolagus cuniculus | |
| 0.376 | - |
phosphate | reaction with guanosine | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| guanosine + phosphate | Oryctolagus cuniculus | enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood | guanine + D-ribose 1-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone marrow | - |
Oryctolagus cuniculus | - |
| intestinal mucosa | - |
Oryctolagus cuniculus | - |
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 4.87 | - |
- |
Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -10 C, stable for 1 month | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxyguanosine + phosphate | - |
Oryctolagus cuniculus | guanine + deoxyribose 1-phosphate | - |
? | |
| guanosine + phosphate | equilibrium constant is 0.019 | Oryctolagus cuniculus | guanine + D-ribose 1-phosphate | - |
r | |
| guanosine + phosphate | enzyme may play an important role in the utilization by bone marrow of the corresponding preformed base and nucleoside reaching this tissue via blood | Oryctolagus cuniculus | guanine + D-ribose 1-phosphate | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
phosphorolysis of deoxyguanosine | Oryctolagus cuniculus |
| 7 | 7.4 | phosphorolysis of guanosine | Oryctolagus cuniculus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8.5 | pH 5.0: about 35% of activity maximum, pH 8.5: about 45% of activity maximum | Oryctolagus cuniculus |
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Release 2023.1 (January 2023)
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