Cloned (Comment) | Organism |
---|---|
expression of wild-type, Y74A, Y258A, Y258F, K326Q, Y329A, G331I, N351A and Y465A mutant enzyme in Escherichia coli | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
G331I | 50% of wild-type glutaminase activity, reduced inhibition by GMP, enhanced inhibition by AMP | Escherichia coli |
K326Q | similar glutaminase and amidotransferase activity as wild-type, not inhibited by GMP | Escherichia coli |
N351A | approx. 50% of wild-type glutaminase activity, completely insensitive to inhibition by GMP, partially resistent to inhibition by AMP | Escherichia coli |
Y258A | complete loss of glutaminase and amidotransferase activity | Escherichia coli |
Y258F | approx. 50% loss of glutaminase activity, very weak amidotransferase activity | Escherichia coli |
Y329A | normal glutaminase activity, approx. 20% amidotransferase activity, less sensitive to GMP inhibition than wild-type | Escherichia coli |
Y465A | 100% of wild-type glutaminase activity, 28% of wild-type amidotransferase activity, inhibition by GMP and AMP is similar to wild-type | Escherichia coli |
Y74A | complete loss of glutaminase activity, little loss of amidotransferase activity | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5'-p-fluorosulfonylbenzoyladenosine | inactivation follows pseudo-first order kinetic, AMP, GMP and 5-phospho-alpha-D-ribose 1-diphosphate protect | Escherichia coli | |
AMP | 4.7 mM, 50% inhibition | Escherichia coli | |
GMP | 1.2-1.4 mM, 50% inhibition | Escherichia coli | |
additional information | synergistic inhibition of glutaminase activity by AMP plus GMP and N3-AMP plus GMP | Escherichia coli | |
N3-AMP | 1.2-1.4 mM, 50% inhibition, AMP and GMP protect | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.39 | - |
5'-p-fluorosulfonylbenzoyladenosine | - |
Escherichia coli |