Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Mg-phosphoribosyldiphosphate | - |
Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | 1.8 mM, 50% inhibition | Homo sapiens | |
GMP | 0.5 mM; 50% inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
L-glutamine | - |
Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens | |
Mg2+ | maximal activation at concentrations 2.5-5 times higher than that of the corresponding 5-phospho-alpha-D-ribose 1-diphosphate concentration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
lymphoblast | diploid wild 2 line, tissue culture | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00125 | 0.0014 | - |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C or 4°C, cell-free extract, overnight, more than 50% loss of activity | Homo sapiens |
-20°C, crude, more than 50% loss of activity overnight | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O | - |
Homo sapiens | 5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
at least 15 min stable | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | 7.4 | Tris/HCl buffer preferred | Homo sapiens |