Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.2.12 extracted from

  • Garten, A.; Petzold, S.; Barnikol-Oettler, A.; Koerner, A.; Thasler, W.E.; Kratzsch, J.; Kiess, W.; Gebhardt, R.
    Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes (2010), Biochem. Biophys. Res. Commun., 391, 376-381.
    View publication on PubMed

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
gel filtration, part of the enzyme in supernatant of HepG2 cells Homo sapiens
120000
-
gel filtration, most of the enzyme in supernatant of HepG2 cells Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
Hep-G2 cell release of NAMPT enzyme into the cell culture supernatant occurs constitutively. HepG2 lysates and supernatants primarily contain the dimeric form of NAMPT which exhibit similar in vitro specific enzymatic activity Homo sapiens
-
hepatocyte release of NAMPT enzyme into the cell culture supernatant occurs constitutively. In primary hepatocytes, secretion within 24 h is far higher than the cellular content, but is neither influenced by inhibitors of secretion nor by glucose, insulin or TNFalpha. In primary human hepatocytes, secreted NAMPT is less active than intracellular enzyme Homo sapiens
-

Subunits

Subunits Comment Organism
dimer 2 * 55000, SDS-PAGE, plus some monomer, supernatant of HepG2 cells Homo sapiens
monomer 1 * 55000, SDS-PAGE, plus some dimer, supernatant of HepG2 cells Homo sapiens