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Literature summary for 2.4.2.12 extracted from
- The NAD biosynthesis pathway mediated by nicotinamide phosphoribosyltransferase regulates Sir2 activity in mammalian cells (2004), J. Biol. Chem., 279, 50754-50763.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, phylogenetic analysis, expression of His-tagged enzyme and of enzyme fused to Sir2 in Escherichia coli strain BL21(DE3) | Mus musculus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.92 | - |
nicotinamide | pH 7.4, 37°C, recombinant enzyme | Mus musculus |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | Mus musculus | key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview | nicotinamide D-ribonucleotide + diphosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q99KQ4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| fibroblast | enzyme and Sir2 expression levels | Mus musculus | - |
| liver | - |
Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | pathway reconstitution in vitro using recombinant enzymes, overview | Mus musculus | ? | - |
? | |
| nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Mus musculus | nicotinamide D-ribonucleotide + diphosphate | - |
? | |
| nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | key enzyme in the regulation of NAD+ biosynthesis, regulates the silent regulator protein 2, i.e. Sir2, activity, the enzyme has a metabolic regulatory function by up- and downregulation of proteins, overview | Mus musculus | nicotinamide D-ribonucleotide + diphosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Nampt | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
nicotinamide | pH 7.4, 37°C, recombinant enzyme | Mus musculus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
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Release 2023.1 (January 2023)
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