Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 50% activation, 1 mM | Rattus norvegicus |
General Stability | Organism |
---|---|
bovine serum albumin, glutathione, cysteine, DTT, EDTA, cystine or H2O2 does not stabilize during storage | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000067 | - |
nicotinamide | - |
Rattus norvegicus | |
0.0038 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Rattus norvegicus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | Rattus norvegicus | first step in NAD synthesis from nicotinamide | nicotinamide D-ribonucleotide + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
partial | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000225 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-20°C to +20°C, about 70% loss of activity overnight | Rattus norvegicus |
storage at room temperature, 4°C, or frozen, unstable upon | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Rattus norvegicus | nicotinamide D-ribonucleotide + diphosphate | - |
? | |
nicotinamide + 5-phospho-alpha-D-ribose 1-diphosphate | first step in NAD synthesis from nicotinamide | Rattus norvegicus | nicotinamide D-ribonucleotide + diphosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | assay at pH 8.8. where NAD glycohydrolase is not active | Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.8 | 9.2 | about half-maximal activity at pH 7.8 and about 80% of maximal activity at pH 9.2 | Rattus norvegicus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | stable | Rattus norvegicus |
8.8 | - |
rapid inactivation below | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | requirement | Rattus norvegicus |