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Literature summary for 2.4.2.10 extracted from
- Orotate phosphoribosyltransferase from Corynebacterium ammoniagenes lacking a conserved lysine (2007), J. Bacteriol., 189, 9030-9036.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DH5alpha cells | Corynebacterium ammoniagenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.033 | - |
Orotate | - |
Corynebacterium ammoniagenes |  |
| 0.036 | - |
diphosphate | - |
Corynebacterium ammoniagenes | |
| 0.045 | - |
orotidine 5'-phosphate | - |
Corynebacterium ammoniagenes | |
| 0.064 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Corynebacterium ammoniagenes | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | Mg2+ is necessary for the activity of the enzyme and can be substituted for by Mn2+ and Co2+ | Corynebacterium ammoniagenes | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21300 | - |
2 * 21300, SDS-PAGE | Corynebacterium ammoniagenes |
| 45400 | - |
native enzyme, gel filtration | Corynebacterium ammoniagenes |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium ammoniagenes | - |
strain ATCC 6872 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation, DEAE-Sepharose Fast Flow column chromatography, and Superdex-75 gel filtration | Corynebacterium ammoniagenes |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24 | - |
recombinant enzyme from crude cell extract, in the presence of Mg2+ | Corynebacterium ammoniagenes |
| 71 | - |
recombinant enzyme after 17fold purification, in the presence of Co2+ | Corynebacterium ammoniagenes |
| 225 | - |
recombinant enzyme after 17fold purification, in the presence of Mn2+ | Corynebacterium ammoniagenes |
| 423 | - |
recombinant enzyme after 17fold purification, in the presence of Mg2+ | Corynebacterium ammoniagenes |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 50 mM Tris-HCl, 2 mM EDTA, 2 mM mercaptoethanol, pH 6.4, several months, no apparent loss of activity | Corynebacterium ammoniagenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| orotate + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Corynebacterium ammoniagenes | orotidine 5'-phosphate + diphosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 21300, SDS-PAGE | Corynebacterium ammoniagenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| OPRTase | - |
Corynebacterium ammoniagenes |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
OPRTase activity decreases with the increase in incubation temperature and more than 50% of the activity is lost upon incubation at 50°C for 30 min | Corynebacterium ammoniagenes |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | reverse (pyrophosphorolysis) reaction | Corynebacterium ammoniagenes |
| 10.5 | 11.5 | forward (phosphoribosyl transfer) reaction | Corynebacterium ammoniagenes |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Corynebacterium ammoniagenes | predicted from amino acid sequence | - |
5.4 |
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