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Literature summary for 2.4.2.10 extracted from

  • Bayles, D.O.; Fennington, G.J., Jr.; Hughes, T.A.
    Sequence and phylogenetic analysis of the Rhizobium leguminosarum biovar trifolii pyrE gene, overproduction, purification and characterization of orotate phosphoribosyltransferase (1997), Gene, 195, 329-336.
    View publication on PubMed
Search for more literature for 2.4.2.10

Cloned(Commentary)

Cloned (Comment) Organism
structural gene pyrE Rhizobium leguminosarum

Inhibitors

Inhibitors Comment Organism Structure
Orotate above 0.4 mM Rhizobium leguminosarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0276
-
 Orotate
-
 Rhizobium leguminosarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24700
-
 x * 24700, SDS-PAGE, recombinant enzyme Rhizobium leguminosarum

Organism

Organism UniProt Comment Textmining
Rhizobium leguminosarum
-
 biovar trifolium
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Rhizobium leguminosarum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
61
-
-
 Rhizobium leguminosarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Rhizobium leguminosarum orotidine 5'-phosphate + diphosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 24700, SDS-PAGE, recombinant enzyme Rhizobium leguminosarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
 recombinant enzyme Rhizobium leguminosarum