Cloned (Comment) | Organism |
---|---|
structural gene pyrE, subcloned from a genomic library in pBR328(pAV002), transduced into pyr-auxotroph Salmonella typhimurium strain SA2434 with phage P22, finally cloned to and expressed in overproducing strain Escherichia coli MB13 | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-phospho-alpha-D-ribose 1-diphosphate | phosphorolysis, product inhibition, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
diphosphate | product inhibition, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
Orotate | phosphorolysis, product inhibition, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium | |
orotidylate | product inhibition, kinetics | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.0031 | 0.0036 | orotidine 5'-phosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
0.044 | - |
5-phospho-alpha-D-ribose 1-diphosphate | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23000 | - |
2 * 23000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | Salmonella enterica subsp. enterica serovar Typhimurium | involved in de novo synthesis of pyrimidine nucleotides | orotidine 5'-phosphate + diphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Salmonella enterica subsp. enterica serovar Typhimurium | - |
structural gene pyrE cloned and overexpressed via multi-copy plasmid in Escherichia coli strain MB13 | - |
Purification (Comment) | Organism |
---|---|
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate | mechanism | Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
95.7 | - |
- |
Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
orotate + 5-phospho-alpha-D-ribose 1-diphosphate | involved in de novo synthesis of pyrimidine nucleotides | Salmonella enterica subsp. enterica serovar Typhimurium | orotidine 5'-phosphate + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 23000, SDS-PAGE | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |