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Literature summary for 2.4.2.10 extracted from

  • Bhatia, M.B.; Vinitsky, A.; Grubmeyer, C.
    Kinetic mechanism of orotate phosphoribosyltransferase from Salmonella typhimurium (1990), Biochemistry, 29, 10480-10487.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
structural gene pyrE, subcloned from a genomic library in pBR328(pAV002), transduced into pyr-auxotroph Salmonella typhimurium strain SA2434 with phage P22, finally cloned to and expressed in overproducing strain Escherichia coli MB13 Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

Inhibitors Comment Organism Structure
5-phospho-alpha-D-ribose 1-diphosphate phosphorolysis, product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
diphosphate product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
Orotate phosphorolysis, product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium
orotidylate product inhibition, kinetics Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.0031 0.0036 orotidine 5'-phosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium
0.044
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
23000
-
2 * 23000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate Salmonella enterica subsp. enterica serovar Typhimurium involved in de novo synthesis of pyrimidine nucleotides orotidine 5'-phosphate + diphosphate
-
?

Organism

Organism UniProt Comment Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
structural gene pyrE cloned and overexpressed via multi-copy plasmid in Escherichia coli strain MB13
-

Purification (Commentary)

Purification (Comment) Organism
-
Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

Reaction Comment Organism Reaction ID
orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate mechanism Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
95.7
-
-
Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
orotate + 5-phospho-alpha-D-ribose 1-diphosphate involved in de novo synthesis of pyrimidine nucleotides Salmonella enterica subsp. enterica serovar Typhimurium orotidine 5'-phosphate + diphosphate
-
?

Subunits

Subunits Comment Organism
dimer 2 * 23000, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Salmonella enterica subsp. enterica serovar Typhimurium