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Literature summary for 2.4.1.B72 extracted from

  • Hoppe, C.M.; Albuquerque-Wendt, A.; Bandini, G.; Leon, D.R.; Shcherbakova, A.; Buettner, F.F.R.; Izquierdo, L.; Costello, C.E.; Bakker, H.; Routier, F.H.
    Apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family (2018), Glycobiology, 28, 333-343 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Caenorhabditis elegans
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Plasmodium falciparum
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells Toxoplasma gondii

Inhibitors

Inhibitors Comment Organism Structure
Amphomycin
-
Plasmodium falciparum
Amphomycin
-
Toxoplasma gondii

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Caenorhabditis elegans
-
-
microsome
-
Plasmodium falciparum
-
-
microsome
-
Toxoplasma gondii
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Caenorhabditis elegans
Mg2+ required Plasmodium falciparum
Mg2+ required Toxoplasma gondii
Mn2+ required Caenorhabditis elegans
Mn2+ required Plasmodium falciparum
Mn2+ required Toxoplasma gondii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Caenorhabditis elegans the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide ?
-
-
additional information Plasmodium falciparum the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide ?
-
-
additional information Toxoplasma gondii the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-
additional information Toxoplasma gondii VEG the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-
additional information Toxoplasma gondii ATCC 50861 the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated ?
-
-

Organism

Organism UniProt Comment Textmining
Caenorhabditis elegans P34413
-
-
Plasmodium falciparum C0H4S1
-
-
Toxoplasma gondii B9QPM1
-
-
Toxoplasma gondii ATCC 50861 B9QPM1
-
-
Toxoplasma gondii VEG B9QPM1
-
-

Source Tissue

Source Tissue Comment Organism Textmining
tachyzoite
-
Toxoplasma gondii
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Ac-WAEW-NH2 peptide + GDP-mannose
-
Caenorhabditis elegans ?
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
-
Plasmodium falciparum ?
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
-
Toxoplasma gondii ?
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
-
Toxoplasma gondii VEG ?
-
?
Ac-WAEW-NH2 peptide + GDP-mannose
-
Toxoplasma gondii ATCC 50861 ?
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
Caenorhabditis elegans ?
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
Plasmodium falciparum ?
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
Toxoplasma gondii ?
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
Toxoplasma gondii VEG ?
-
?
Ac-WAEWGEC-NH2 peptide + GDP-mannose
-
Toxoplasma gondii ATCC 50861 ?
-
?
additional information the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide Caenorhabditis elegans ?
-
-
additional information the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide Plasmodium falciparum ?
-
-
additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii ?
-
-
additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii VEG ?
-
-
additional information the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated Toxoplasma gondii ATCC 50861 ?
-
-

Synonyms

Synonyms Comment Organism
DPY-19
-
Caenorhabditis elegans
DPY-19
-
Plasmodium falciparum
DPY-19
-
Toxoplasma gondii
Dpy-19-like C-mannosyltransferase UniProt Plasmodium falciparum
dpy19
-
Caenorhabditis elegans
dpy19
-
Plasmodium falciparum
dpy19
-
Toxoplasma gondii
Dumpy-19 protein homologue UniProt Toxoplasma gondii
protein dumpy-19 UniProt Caenorhabditis elegans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Caenorhabditis elegans
37
-
assay at Plasmodium falciparum
37
-
assay at Toxoplasma gondii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Caenorhabditis elegans
7.5
-
assay at Plasmodium falciparum
7.5
-
assay at Toxoplasma gondii

General Information

General Information Comment Organism
malfunction a DPY-19 mutant shows a dumpy phenotype and a defect in Q neuroblast migration Caenorhabditis elegans
physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family Caenorhabditis elegans
physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Plasmodium falciparum Dpy19 is a C-mannosyltransferase Plasmodium falciparum
physiological function apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase Toxoplasma gondii