Cloned (Comment) | Organism |
---|---|
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells | Caenorhabditis elegans |
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells | Plasmodium falciparum |
gene dpy19, recombinant enzyme expression in DPY19L1/L3/L4- CHO cells | Toxoplasma gondii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Amphomycin | - |
Plasmodium falciparum | |
Amphomycin | - |
Toxoplasma gondii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Caenorhabditis elegans | - |
- |
microsome | - |
Plasmodium falciparum | - |
- |
microsome | - |
Toxoplasma gondii | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Caenorhabditis elegans | |
Mg2+ | required | Plasmodium falciparum | |
Mg2+ | required | Toxoplasma gondii | |
Mn2+ | required | Caenorhabditis elegans | |
Mn2+ | required | Plasmodium falciparum | |
Mn2+ | required | Toxoplasma gondii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Caenorhabditis elegans | the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide | ? | - |
- |
|
additional information | Plasmodium falciparum | the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide | ? | - |
- |
|
additional information | Toxoplasma gondii | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | ? | - |
- |
|
additional information | Toxoplasma gondii VEG | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | ? | - |
- |
|
additional information | Toxoplasma gondii ATCC 50861 | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caenorhabditis elegans | P34413 | - |
- |
Plasmodium falciparum | C0H4S1 | - |
- |
Toxoplasma gondii | B9QPM1 | - |
- |
Toxoplasma gondii ATCC 50861 | B9QPM1 | - |
- |
Toxoplasma gondii VEG | B9QPM1 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
tachyzoite | - |
Toxoplasma gondii | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Ac-WAEW-NH2 peptide + GDP-mannose | - |
Caenorhabditis elegans | ? | - |
? | |
Ac-WAEW-NH2 peptide + GDP-mannose | - |
Plasmodium falciparum | ? | - |
? | |
Ac-WAEW-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii | ? | - |
? | |
Ac-WAEW-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii VEG | ? | - |
? | |
Ac-WAEW-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii ATCC 50861 | ? | - |
? | |
Ac-WAEWGEC-NH2 peptide + GDP-mannose | - |
Caenorhabditis elegans | ? | - |
? | |
Ac-WAEWGEC-NH2 peptide + GDP-mannose | - |
Plasmodium falciparum | ? | - |
? | |
Ac-WAEWGEC-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii | ? | - |
? | |
Ac-WAEWGEC-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii VEG | ? | - |
? | |
Ac-WAEWGEC-NH2 peptide + GDP-mannose | - |
Toxoplasma gondii ATCC 50861 | ? | - |
? | |
additional information | the enzyme can transfer mannose to adhesive thrombospondin type 1 repeats (TSRs) and type I cytokine receptors. Enzyme Dpy19 of Caenorhabditis elegans can transfer mannose to a short WXXW peptide | Caenorhabditis elegans | ? | - |
- |
|
additional information | the micronemal adhesin thrombospondin-related anonymous protein (TRAP) is C-hexosylated in Plasmodium falciparum sporozoites. When expressed in a mammalian cell line deficient in C-mannosylation, the Plasmodium falciparum Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide | Plasmodium falciparum | ? | - |
- |
|
additional information | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | Toxoplasma gondii | ? | - |
- |
|
additional information | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | Toxoplasma gondii VEG | ? | - |
- |
|
additional information | the micronemal protein MIC2 secreted by Toxoplasma gondii tachyzoites is C-hexosylated. When expressed in a mammalian cell line deficient in C-mannosylation, the Toxoplasma gondii Dpy19 homologue is able to modify TSR domains of the micronemal adhesins TRAP/MIC2 family involved in parasite motility and invasion. In vitro, the apicomplexan enzyme can transfer mannose to a WXXWXXC peptide but, in contrast to Caenorhabditis elegans or mammalian C-mannosyltransferases, is inactive on a short WXXW peptide. MIC2 secreted by Toxoplasma tachyzoites is C-mannosylated | Toxoplasma gondii ATCC 50861 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DPY-19 | - |
Caenorhabditis elegans |
DPY-19 | - |
Plasmodium falciparum |
DPY-19 | - |
Toxoplasma gondii |
Dpy-19-like C-mannosyltransferase | UniProt | Plasmodium falciparum |
dpy19 | - |
Caenorhabditis elegans |
dpy19 | - |
Plasmodium falciparum |
dpy19 | - |
Toxoplasma gondii |
Dumpy-19 protein homologue | UniProt | Toxoplasma gondii |
protein dumpy-19 | UniProt | Caenorhabditis elegans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Caenorhabditis elegans |
37 | - |
assay at | Plasmodium falciparum |
37 | - |
assay at | Toxoplasma gondii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Caenorhabditis elegans |
7.5 | - |
assay at | Plasmodium falciparum |
7.5 | - |
assay at | Toxoplasma gondii |
General Information | Comment | Organism |
---|---|---|
malfunction | a DPY-19 mutant shows a dumpy phenotype and a defect in Q neuroblast migration | Caenorhabditis elegans |
physiological function | apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family | Caenorhabditis elegans |
physiological function | apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Plasmodium falciparum Dpy19 is a C-mannosyltransferase | Plasmodium falciparum |
physiological function | apicomplexan C-mannosyltransferases modify thrombospondin type I-containing adhesins of the TRAP family. Toxoplasma gondii Dpy19 is a C-mannosyltransferase | Toxoplasma gondii |