Cloned (Comment) | Organism |
---|---|
recombinant expression in S2 cells, coexpression with substrate Rspo1 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme knockdown by siRNA | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q6ZPD9 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, coexpressed with substrate Rspo1, by heparin and nickel affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156 | Homo sapiens | ? | - |
? | |
additional information | C-mannosylation of protein Rspo1, Rspo1 has two predicted C-mannosylation sites in the thrombospondin type 1 repeat TSR1, substrate structure, overview. Enzyme DPY19L3 selectively modified Rspo1 at residue W156 but not W153 based on mass spectrometry. Purified recombinant Rspo1 protein from the conditioned medium of overexpressing HT1080-Rspo1-MH cells | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C-mannosyltransferase | - |
Homo sapiens |
DPY19L3 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | knockdown of DPY19L3 inhibits the secretion of Rspo1. Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity | Homo sapiens |
physiological function | DPY19L3-mediated C-mannosylation of Rspo1 at W156 is required for its secretion. R-spondin1 (Rspo1) is a secreted protein that enhances Wnt signaling, which has crucial functions in embryonic development and several cancers. C-mannosylation is a posttranslational modification of the first tryptophan residue in the consensus sequence W-X-X-W/C (in which X represents any amino acid) by an endoplasmic reticulum-localized enzyme. C-mannosylation is important for proteinprotein interactions. Enhancement of canonical Wnt signaling by Rspo1 is regulated by C-mannosylation | Homo sapiens |