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Literature summary for 2.4.1.B72 extracted from

  • Niwa, Y.; Suzuki, T.; Dohmae, N.; Simizu, S.
    Identification of DPY19L3 as the C-mannosyltransferase of R-spondin1 in human cells (2016), Mol. Biol. Cell, 27, 744-756.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression in S2 cells, coexpression with substrate Rspo1 Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information enzyme knockdown by siRNA Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156 ?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6ZPD9
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme, coexpressed with substrate Rspo1, by heparin and nickel affinity chromatography Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
additional information Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity Homo sapiens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DPY19L3 is the C-mannosyltransferase of Rspo1 at residue W156 Homo sapiens ?
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?
additional information C-mannosylation of protein Rspo1, Rspo1 has two predicted C-mannosylation sites in the thrombospondin type 1 repeat TSR1, substrate structure, overview. Enzyme DPY19L3 selectively modified Rspo1 at residue W156 but not W153 based on mass spectrometry. Purified recombinant Rspo1 protein from the conditioned medium of overexpressing HT1080-Rspo1-MH cells Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
C-mannosyltransferase
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Homo sapiens
DPY19L3
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Homo sapiens

General Information

General Information Comment Organism
malfunction knockdown of DPY19L3 inhibits the secretion of Rspo1. Lec15.2 cells lack dolichol-phosphate-mannose synthesis activity Homo sapiens
physiological function DPY19L3-medi­ated C-mannosylation of Rspo1 at W156 is required for its secretion. R-spondin1 (Rspo1) is a secreted protein that enhances Wnt signaling, which has crucial functions in embryonic development and several cancers. C-mannosylation is a posttranslational modification of the first tryptophan residue in the consensus sequence W-X-X-W/C (in which X represents any amino acid) by an endoplasmic reticulum-lo­calized enzyme. C-mannosylation is important for protein–protein interactions. Enhancement of canonical Wnt signaling by Rspo1 is regulated by C-mannosylation Homo sapiens