Literature summary for 2.4.1.B3 extracted from

Vos, A.; Dekker, N.; Distel, B.; Leunissen, J.A.; Hochstenbach, F.
Role of the synthase domain of Ags1p in cell wall alpha-glucan biosynthesis in fission yeast (2007), J. Biol. Chem., 282, 18969-18979.

Search for more literature for 2.4.1.B3

Cloned(Commentary)

Cloned (Comment)	Organism
expression of native and E1526A mutant enzyme in Schizosaccharomyces pombe, overexpression of native but not mutant enzyme results in accumulation of (1,4)-alpha-glucan	Schizosaccharomyces pombe

Protein Variants

Protein Variants	Comment	Organism
DELTA1118-1689	enzyme is not able to synthesize alpha-1,4-glucan	Schizosaccharomyces pombe
DELTA113-724	enzyme is able to synthesize alpha-1,4-glucan	Schizosaccharomyces pombe
E1526A	markedly reduced ability to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant, no accumulation of alpha-1,4-glucan	Schizosaccharomyces pombe
E1534A	able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant	Schizosaccharomyces pombe
G1165A	able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant	Schizosaccharomyces pombe
G696S	temperature-sensitive mutation, gene is functional at 28°C but not at 36°C	Schizosaccharomyces pombe
K1163Q	able to complement the cell lysis phenotype and temperature sensitivity of the Schizosaccharomyces pombe ags1-1 mutant	Schizosaccharomyces pombe
K1422Q	reduced accumulation of alpha-1,4-glucan	Schizosaccharomyces pombe

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Schizosaccharomyces pombe	16020	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
265000	-	x * 265000, SDS-PAGE	Schizosaccharomyces pombe
272106	-	x * 272106, calculated from the deduced amino acid sequence	Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Schizosaccharomyces pombe	required for the biosynthesis of alpha-1,4-glucan in cell wall alpha-glucan	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Schizosaccharomyces pombe	Q9USK8	study deals with the intracellular domain of the protein	-

Reaction

Reaction	Comment	Organism	Reaction ID
UDP-glucose + [alpha-D-glucosyl-(1-3)]n = UDP + alpha-1,4-glucosyl-[alpha-D-glucosyl-(1-3)]n	adds the approximately 10% of alpha-1,4-linked glucose residues to alpha-1,3-glucan chains	Schizosaccharomyces pombe	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	required for the biosynthesis of alpha-1,4-glucan in cell wall alpha-glucan	Schizosaccharomyces pombe	?	-	?

Subunits

Subunits	Comment	Organism
?	x * 265000, SDS-PAGE	Schizosaccharomyces pombe
?	x * 272106, calculated from the deduced amino acid sequence	Schizosaccharomyces pombe

Synonyms

Synonyms	Comment	Organism
Ags1p	multidomain protein with two probable catalytic domains predicted to reside at opposite sides of the plasma membrane, as well as a multipass transmembrane domain	Schizosaccharomyces pombe
cell wall alpha-1,4-glucan synthase	-	Schizosaccharomyces pombe

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Release 2023.1 (January 2023)