Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CaCl2 | 69% residual activity at 10 mM | Rhodotorula sp. | |
Citric acid | 65% residual activity at 10 mM | Rhodotorula sp. | |
KCl | 40% residual activity at 10 mM | Rhodotorula sp. | |
MnCl2 | 99% residual activity at 10 mM | Rhodotorula sp. | |
NaCl | 74% residual activity at 10 mM | Rhodotorula sp. | |
ZnSO4 | 68% residual activity at 10 mM | Rhodotorula sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Rhodotorula sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | 121% activity at 10 mM | Rhodotorula sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77000 | - |
2 * 77000, SDS-PAGE | Rhodotorula sp. |
170000 | - |
gel filtration | Rhodotorula sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + sucrose | Rhodotorula sp. | - |
D-glucose + 1-kestose | - |
? | |
sucrose + sucrose | Rhodotorula sp. LEB-V10 | - |
D-glucose + 1-kestose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula sp. | - |
- |
- |
Rhodotorula sp. LEB-V10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Rhodotorula sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.5 | - |
crude extract | Rhodotorula sp. |
75.4 | - |
after 150.8fold purification | Rhodotorula sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + sucrose | - |
Rhodotorula sp. | D-glucose + 1-kestose | - |
? | |
sucrose + sucrose | - |
Rhodotorula sp. LEB-V10 | D-glucose + 1-kestose | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 77000, SDS-PAGE | Rhodotorula sp. |
Synonyms | Comment | Organism |
---|---|---|
fructooligosaccharide-producing extracellular enzyme | - |
Rhodotorula sp. |
fructosyl-transferase | - |
Rhodotorula sp. |
FTA | - |
Rhodotorula sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 70 | - |
Rhodotorula sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
very stable at temperatures below 66°C, the half-life of the enzyme decreases very quickly at temperatures above 66°C | Rhodotorula sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
around pH 5.0 | Rhodotorula sp. |