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Literature summary for 2.4.1.99 extracted from

  • Altenbach, D.; Rudino-Pinera, E.; Olvera, C.; Boller, T.; Wiemken, A.; Ritsema, T.
    An acceptor-substrate binding site determining glycosyl transfer emerges from mutant analysis of a plant vacuolar invertase and a fructosyltransferase (2009), Plant Mol. Biol., 69, 47-56.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Pichia pastoris Lolium arundinaceum

Protein Variants

Protein Variants Comment Organism
C203N the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme Lolium arundinaceum
L322F the mutant shows wild type activity Lolium arundinaceum
M173L the mutant shows wild type activity Lolium arundinaceum
W362Y the mutant has an almost doubled relative hydrolytic activity in comparison to wild type enzyme Lolium arundinaceum
Y248V the mutant shows no activity Lolium arundinaceum
Y317W the mutant shows wild type activity Lolium arundinaceum
Y408W the mutant shows low activity Lolium arundinaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.8
-
 sucrose wild type enzyme Lolium arundinaceum

Organism

Organism UniProt Comment Textmining
Lolium arundinaceum Q9FSV7 before known as Festuca arundinacea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 1-SST shows relatively low hydrolase activity at all offered sucrose concentrations as compared to the glycosyltransferase activity Lolium arundinaceum ?
-
 ?
sucrose + sucrose
-
 Lolium arundinaceum D-glucose + 1-kestose
-
 ?

Synonyms

Synonyms Comment Organism
1-SST
-
 Lolium arundinaceum
sucrose:sucrose 1-fructosyltransferase
-
 Lolium arundinaceum