Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Euglena gracilis var. saccharophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
1-deoxynojirimycin | weak competitive inhibition | Euglena gracilis var. saccharophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.56 | - |
alpha-D-glucose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
0.67 | - |
laminaribiose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.26 | - |
laminaritriose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.41 | - |
laminaritetraose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
2.29 | - |
Laminaripentaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
2.88 | - |
Laminarihexaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Euglena gracilis var. saccharophila | A0A2I6SUQ9 | glycoside hydrolase family GH149 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-D-glucose + glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | phosphate + laminaribiose | - |
r | |
laminaribiose + glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | phosphate + laminaritriose | - |
r | |
laminarihexaose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | laminariheptaose + phosphate | - |
r | |
laminaripentaose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | laminarihexaose + phosphate | - |
r | |
laminaritetraose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | laminaripentaose + phosphate | - |
r | |
laminaritriose + alpha-D-glucose 1-phosphate | - |
Euglena gracilis var. saccharophila | laminaritetraose + phosphate | - |
r | |
laminaritriose + phosphate | - |
Euglena gracilis var. saccharophila | laminaribiose + alpha-D-glucose 1-phosphate | - |
? | |
additional information | protein is able to catalyze the conversion of glucose and glucose 1-phosphate into oligosaccharides with degree of polymerization up to 12 glucose units. Tps3 acts exclusively on glucose-beta-1,3-glucose oligosaccharides.The activity is dependent on both glucose and glucose 1-phosphate as substrates, excluding either from the reaction mixture completely abolishes oligosaccharide production | Euglena gracilis var. saccharophila | ? | - |
- |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 40 | - |
Euglena gracilis var. saccharophila |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.08 | - |
laminaribiose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.1 | - |
alpha-D-glucose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.1 | - |
Laminarihexaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.12 | - |
laminaritriose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.12 | - |
laminaritetraose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.13 | - |
Laminaripentaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 8 | - |
Euglena gracilis var. saccharophila |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
Laminarihexaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
0.5 | - |
Laminaripentaose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
0.79 | - |
laminaritetraose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
0.89 | - |
laminaritriose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.62 | - |
laminaribiose | pH 7.0, 30°C | Euglena gracilis var. saccharophila | |
1.99 | - |
alpha-D-glucose | pH 7.0, 30°C | Euglena gracilis var. saccharophila |