Cloned (Comment) | Organism |
---|---|
enzyme expression analysis in larvae, expression of the FLAG-tagged catalytic domain, comprisisng residues 62403, in the Spodoptera frugiperda Sf21 cell baculovirus expression system | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
additional information | enzyme activity in beta4GalNAcT RNAi flies is reduced | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Drosophila melanogaster | the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged catalytic domain from Spodoptera frugiperda Sf21 cells by anti-FLAG M1 affinity chromatography | Drosophila melanogaster |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
larva | third instar larvae | Drosophila melanogaster | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure | Drosophila melanogaster | ? | - |
? | |
additional information | no activity with donor UDP-galactose, the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, such as GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo | Drosophila melanogaster | ? | - |
? | |
UDP-GalNAc + GlcNAcbeta1,3Fuc | DNS-labeled acceptor substrate | Drosophila melanogaster | UDP + GalNAcbeta1,4GlcNAcbeta1,3Fuc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta 1,4-N-acetylgalactosaminyltransferase-A | - |
Drosophila melanogaster |
beta4GalNAcT | - |
Drosophila melanogaster |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Drosophila melanogaster |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.4 | assay at | Drosophila melanogaster |