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Literature summary for 2.4.1.92 extracted from

  • Sasaki, N.; Yoshida, H.; Fuwa, T.J.; Kinoshita-Toyoda, A.; Toyoda, H.; Hirabayashi, Y.; Ishida, H.; Ueda, R.; Nishihara, S.
    Drosophila beta1,4-N-acetylgalactosaminyltransferase-A synthesizes the LacdiNAc structures on several glycoproteins and glycosphingolipids (2007), Biochem. Biophys. Res. Commun., 354, 522-527.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
enzyme expression analysis in larvae, expression of the FLAG-tagged catalytic domain, comprisisng residues 62–403, in the Spodoptera frugiperda Sf21 cell baculovirus expression system Drosophila melanogaster

Protein Variants

Protein Variants Comment Organism
additional information enzyme activity in beta4GalNAcT RNAi flies is reduced Drosophila melanogaster

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Drosophila melanogaster

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Drosophila melanogaster the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure ?
-
?

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged catalytic domain from Spodoptera frugiperda Sf21 cells by anti-FLAG M1 affinity chromatography Drosophila melanogaster

Source Tissue

Source Tissue Comment Organism Textmining
larva third instar larvae Drosophila melanogaster
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Drosophila melanogaster

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo, the GalNAcbeta1,4GlcNAc, i.e. LacdiNAc or LDN, structure is a more common structural feature in invertebrate glycoconjugates when compared with the Galbeta1,4GlcNAc, i.e. LacNAc or LN, structure Drosophila melanogaster ?
-
?
additional information no activity with donor UDP-galactose, the enzyme synthesizes the LacdiNAc structures, with GalNAcbeta1,4GlcNAc, on several glycoproteins and glycosphingolipids, including N-glycans, O-glycans, and the arthro-series GSLs, such as GalNAcbeta1,4Glc-NAcbeta1,3Manbeta1,4Glcbeta1Cer, and neutral GSLs, in vitro and in vivo Drosophila melanogaster ?
-
?
UDP-GalNAc + GlcNAcbeta1,3Fuc DNS-labeled acceptor substrate Drosophila melanogaster UDP + GalNAcbeta1,4GlcNAcbeta1,3Fuc
-
?

Synonyms

Synonyms Comment Organism
beta 1,4-N-acetylgalactosaminyltransferase-A
-
Drosophila melanogaster
beta4GalNAcT
-
Drosophila melanogaster

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Drosophila melanogaster

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.4 assay at Drosophila melanogaster