Any feedback?
Literature summary for 2.4.1.91 extracted from
- Molecular cloning and biochemical characterization of the UDP-glucose: flavonoid 3-O-beta-D-glucosyltransferase from Concord grape (Vitis labrusca) (2012), Phytochemistry, 74, 90-99.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli DE3 pLys S cells | Vitis labrusca |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00216 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca |  |
| 0.00479 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
| 0.914 | - |
UDP-alpha-D-glucose | using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vitis labrusca | A6N928 | cultivar Concord | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| glutathione Sepharose column chromatography | Vitis labrusca |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| exocarp | pre-veraison exocarp | Vitis labrusca | - |
| flower | highest expression | Vitis labrusca | - |
| flower bud | - |
Vitis labrusca | - |
| leaf | - |
Vitis labrusca | - |
| additional information | not detected in mesocarp | Vitis labrusca | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + cyanidin | this enzyme preferentially glucosylates cyanidin as compared with quercetin | Vitis labrusca | UDP + cyanidin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + delphinidin | - |
Vitis labrusca | UDP + delphinidin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + dihydroquercetin | weak substrate | Vitis labrusca | UDP + quercetin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + isorhamnetin | - |
Vitis labrusca | UDP + isorhamnetin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + kaempferol | weak substrate | Vitis labrusca | UDP + kaempferol 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + malvidin | best substrate | Vitis labrusca | UDP + malvidin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + peonidin | - |
Vitis labrusca | UDP + peonidin 3-O-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + quercetin | - |
Vitis labrusca | UDP + quercetin 3-O-beta-D-glucoside | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucose: flavonoid 3-O-glucosyltransferase | - |
Vitis labrusca |
| VL3GT | - |
Vitis labrusca |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000113 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
| 0.000247 | - |
UDP-alpha-D-glucose | using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
| 0.000702 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Vitis labrusca | while there is little enzyme expression before veraison, these transcripts begin to accumulate at week 8 and 10 after flowering to reach maximal levels at week 12 after flowering and decreases thereafter | up |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00027 | - |
UDP-alpha-D-glucose | using cyanidin as cosubstrate, in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
| 0.052 | - |
quercetin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
| 0.146 | - |
cyanidin | in 100 mM Tris-HCl pH 8.0, at 30°C | Vitis labrusca | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
