Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3)RIL cells | Citrus x paradisi |
General Stability | Organism |
---|---|
activities recorded in phosphate, MOPS, and bicine buffers are not significantly different, but assays in Tris buffer result in a reduction of enzyme activity by 51% under optimal pH conditions | Citrus x paradisi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2,3-Butanedione | 46% residual activity at 10 mM | Citrus x paradisi | |
Cu2+ | 0.03% residual activity at 10 mM | Citrus x paradisi | |
Fe2+ | 0.09% residual activity at 10 mM | Citrus x paradisi | |
additional information | L-cysteine, L-histidine, L-arginine, L-tryptophan, and L-tyrosine residues are important for activity; not inhibited by succinic anhydride, K+, Na+, Ca2+,Mg2+, and Mn2+ | Citrus x paradisi | |
N-bromo-succinimide | 17% residual activity at 10 mM | Citrus x paradisi | |
Tetranitromethane | 13% residual activity at 10 mM | Citrus x paradisi | |
Tris | Tris inhibits flavonol 3-O-GT as activity levels are significantly lower than corresponding buffers across its entire buffer range | Citrus x paradisi | |
UDP | 6% residual activity at 10 mM | Citrus x paradisi | |
Zn2+ | 26% residual activity at 10 mM | Citrus x paradisi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.012 | - |
kaempferol | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
0.033 | - |
myricetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
0.067 | - |
quercetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
0.669 | - |
UDP-glucose | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 119% relative activity at 1 mM | Citrus x paradisi | |
Na2SO4 | 118% relative activity at 1 mM | Citrus x paradisi | |
Succinic anhydride | 115% relative activity at 1 mM | Citrus x paradisi |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
SDS-PAGE | Citrus x paradisi |
51200 | - |
calculated from amino acid sequence | Citrus x paradisi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus x paradisi | C5MR71 | - |
- |
Purification (Comment) | Organism |
---|---|
TALON metal affinity resin column chromatography, gel filtration | Citrus x paradisi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | does not glucosylate naringenin, apigenin, luteolin, dihydroquercetin, 4'-acetoxy-7-hydroxy-6-methoxy-isoflavone, 4'-methoxy-flavonol, 3',4'-dimethoxy-flavonol, anthocyanidin, and cyanidin | Citrus x paradisi | ? | - |
? | |
UDP-alpha-D-glucose + kaempferol | 57.9% activity compared to quercetin, glucosylation occurs at the 3 hydroxyl position | Citrus x paradisi | UDP + kaempferol 3-O-beta-D-glucoside | - |
? | |
UDP-alpha-D-glucose + quercetin | 100% activity, glucosylation occurs at the 3 hydroxyl position | Citrus x paradisi | UDP + quercetin 3-O-beta-D-glucoside | - |
ir | |
UDP-glucose + myricetin | 40.7% activity compared to quercetin, glucosylation occurs at the 3 hydroxyl position | Citrus x paradisi | UDP + myricetin 3-O-beta-D-glucoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
flavonol 3-O-glucosyltransferase | - |
Citrus x paradisi |
flavonol 3-O-GT | - |
Citrus x paradisi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Citrus x paradisi |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 60 | the enzyme is stable when preincubated for up to 10 min at 40°C with a significant decrease in activity at 50°C and an almost complete loss of detectable activity at 60°C and beyond | Citrus x paradisi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5955 | - |
kaempferol | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
0.6252 | - |
myricetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
1.047 | - |
quercetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
1.442 | - |
UDP-glucose | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
the optimum pH is 7.5 with a pronounced buffer effect noted for reactions performed in Tris-HCl buffer | Citrus x paradisi |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | reduction to 59% maximal activity at pH 5.5 and 57% at pH 8.5 | Citrus x paradisi |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Citrus x paradisi | calculated from amino acid sequence | - |
6.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
UDP-glucose | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
15.6 | - |
quercetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
18.9 | - |
myricetin | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi | |
49.6 | - |
kaempferol | apparent value, in 50 mM phosphate buffer (pH 7.5), at 40°C | Citrus x paradisi |