Cloned (Comment) | Organism |
---|---|
- |
Vitis vinifera |
General Stability | Organism |
---|---|
at least three freeze-thaw cycles can be tolerated without apparent loss of activity | Vitis vinifera |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Vitis vinifera | |
Mg2+ | - |
Vitis vinifera | |
Zn2+ | - |
Vitis vinifera |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
quercetin | recombinant enzyme | Vitis vinifera | |
0.016 | - |
delphinidin | recombinant enzyme | Vitis vinifera | |
0.03 | - |
cyanidin | recombinant enzyme | Vitis vinifera | |
0.0357 | - |
malvidin | recombinant enzyme | Vitis vinifera | |
1.9 | - |
UDPglucose | recombinant enzyme | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPglucose + a flavonol | Vitis vinifera | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | UDP + a flavonol 3-O-D-glucoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Vitis vinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Vitis vinifera | - |
leaf | - |
Vitis vinifera | - |
Storage Stability | Organism |
---|---|
-20°C or 4°C, the recombinant enzyme is stable for at least 120 h | Vitis vinifera |
-80°C, stable for many months | Vitis vinifera |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | only UDPglucose can serve as glucosyl donor | Vitis vinifera | ? | - |
? | |
UDP-glucose + delphinidin | - |
Vitis vinifera | UDP + delphinidin 3-O-glucoside | - |
? | |
UDPglucose + a flavonol | the principal, if not only, role of the enzyme is to glucosylate anthocyanidins in red fruit during ripening | Vitis vinifera | UDP + a flavonol 3-O-D-glucoside | - |
? | |
UDPglucose + cyanidin | - |
Vitis vinifera | UDP + cyanidin 3-O-glucoside | - |
? | |
UDPglucose + malvidin | - |
Vitis vinifera | UDP + malvidin 3-O-glucoside | - |
? | |
UDPglucose + quercetin | - |
Vitis vinifera | UDP + quercetin 3-O-glucoside | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
reaction with quercetin or cyanidin | Vitis vinifera |