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Literature summary for 2.4.1.91 extracted from

  • Ishikura, N.; Yang, Z.Q.
    Multiple forms of flavonol O-glucosyltransferases in young leaves of Euonymus alatus f. ciliato-denatus (1994), Phytochemistry, 36, 1139-1145.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 14 mM, activation of isoenzyme F3GT1 to 160%, activation of isoenzyme F3GT2 to 136% Euonymus alatus
EDTA slight activation Euonymus alatus

Inhibitors

Inhibitors Comment Organism Structure
CaCl2 10 mM, 9% inhibition Euonymus alatus
CoCl2 1 mM, 50% inhibition of isoenzyme F3GT1 and 25% inhibition of isoenzyme F3GT2 Euonymus alatus
Cu2+ 1 mM CuCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 Euonymus alatus
dithioerythritol
-
Euonymus alatus
EDTA 10 mM, slight inhibition Euonymus alatus
iodoacetamide
-
Euonymus alatus
iodoacetate
-
Euonymus alatus
KCl 10 mM, 9-10% inhibition Euonymus alatus
Mn2+ 10 mM MnCl2, 52% inhibition of isoenzyme F3GT1 and 28% inhibition of isoenzyme F3GT2 Euonymus alatus
NEM
-
Euonymus alatus
PCMB
-
Euonymus alatus
Phenylmercuriacetate
-
Euonymus alatus
Zn2+ 1 mM ZnCl2, 93% inhibition of isoenzyme F3GT1 and 94% inhibition of isoenzyme F3GT2 Euonymus alatus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ 10 mM, slight stimulatory effect on isoenzyme F3GT1 and F3GT2 Euonymus alatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
gel filtration Euonymus alatus

Organism

Organism UniProt Comment Textmining
Euonymus alatus
-
f. Ciliato-dentatus
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme F3GT1 and F3GT2 Euonymus alatus

Source Tissue

Source Tissue Comment Organism Textmining
leaf young Euonymus alatus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + isorhamnetin
-
Euonymus alatus UDP + isorhamnetin 3-O-beta-D-glucoside
-
?
UDP-alpha-D-glucose + kaempferol 7-O-glucoside isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 5% of the activity with kaempferol Euonymus alatus UDP + kaempferol 3,7-O-diglucoside
-
?
UDP-alpha-D-glucose + quercetin 7-O-beta-D-glucoside isoenzyme F3GT1: 6% of the activity with kaempferol, isoenzyme F3GT2: 6% of the activity with kaempferol Euonymus alatus UDP + quercetin 3,7-O-di-beta-D-glucoside
-
?
UDPglucose + 3,5,7-trihydroxyflavanone isoenzyme F3GT1: 22% of the activity with kaempferol, isoenzyme F3GT2: 12% of the activity with kaempferol Euonymus alatus UDP + 3,5,7-trihydroxyflavanone 3-O-glucoside
-
?
UDPglucose + 7,4'-dihydroxyflavonol isoenzyme F3GT1: 33% of the activity with kaempferol, isoenzyme F3GT2: 34% of the activity with kaempferol Euonymus alatus UDP + 7,4'-dihydroxyflavonol 3-O-glucoside
-
?
UDPglucose + dihydrokaempferol isoenzyme F3GT1: 7% of the activity with kaempferol, isoenzyme F3GT2: 7% of the activity with kaempferol Euonymus alatus UDP + dihydrokaempferol 3-O-glucoside
-
?
UDPglucose + dihydroquercetin isoenzyme F3GT1: 3% of the activity with kaempferol, isoenzyme F3GT2: 2% of the activity with kaempferol Euonymus alatus UDP + dihydroquercetin 3-O-glucoside
-
?
UDPglucose + fisetin
-
Euonymus alatus UDP + fisetin 3-O-glucoside
-
?
UDPglucose + kaempferol
-
Euonymus alatus UDP + kaempferol 3-O-glucoside
-
?
UDPglucose + kaempferol 5,7,4'-trimethyl ether isoenzyme F3GT1: 52% of the activity with kaempferol, isoenzyme F3GT2: 11% of the activity with kaempferol Euonymus alatus UDP + kaempferol 5,7,4'-trimethyl ether 3-O-glucoside
-
?
UDPglucose + kaempferol-4'-O-methylether
-
Euonymus alatus UDP + ?
-
?
UDPglucose + myricetin
-
Euonymus alatus UDP + myrecetin 3-O-glucoside
-
?
UDPglucose + quercetin
-
Euonymus alatus UDP + quercetin 3-O-glucoside
-
?
UDPglucose + quercetin 5-methyl ether isoenzyme F3GT1: 38% of the activity with kaempferol, isoenzyme F3GT2: 25% of the activity with kaempferol Euonymus alatus UDP + quercetin 5-methyl ether 3-O-glucoside
-
?
UDPglucose + rhamnetin isoenzyme F3GT1: 20% of the activity with kaempferol, isoenzyme F3GT2: 82% of the activity with kaempferol Euonymus alatus UDP + rhamnetin 3-O-glucoside
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Euonymus alatus