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Literature summary for 2.4.1.90 extracted from

  • Miyazaki, T.; Miyashita, R.; Nakamura, S.; Ikegaya, M.; Kato, T.; Park, E.Y.
    Biochemical characterization and mutational analysis of silkworm Bombyx mori beta-1,4-N-acetylgalactosaminyltransferase and insight into the substrate specificity of beta-1,4-galactosyltransferase family enzymes (2019), Insect Biochem. Mol. Biol., 115, 103254 .
    View publication on PubMed
Search for more literature for 2.4.1.90

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ less than 4% activity in the presence of Ca2+ions Bombyx mori
Co2+ less than 4% activity in the presence of Co2+ions Bombyx mori
Mg2+ less than 4% activity in the presence of Mg2+ions Bombyx mori

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 100% activity at 1 mM Bombyx mori

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-galactose + N-acetyl-D-glucosamine Bombyx mori
-
 UDP + N-acetyllactosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Bombyx mori G1UHW5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme has 10 N-glycosylation sites Bombyx mori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme does not exhibit beta-1,4-galactosyltransferase activity. No activity with 4-nitrophenyl N-acetyl-beta-D-galactosaminide, 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-galactopyranoside, 4-nitrophenyl alpha-D-mannopyranoside, GalNAcbeta(1->4)GlcNAcbeta-pNP, and Galbeta(1->4)GlcNAcbeta-pNP Bombyx mori ?
-
-
UDP-alpha-D-galactose + 4-nitrophenyl beta-D-glucopyranoside 0.26% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Bombyx mori ?
-
 ?
UDP-alpha-D-galactose + 4-nitrophenyl N-acetyl-beta-D-glucosaminide 100% activity Bombyx mori UDP + 4-nitrophenyl N-acetyllactosamine
-
 ?
UDP-alpha-D-galactose + GlcNAcbeta(1->2)Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA 61% activity compared to Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA Bombyx mori ?
-
 ?
UDP-alpha-D-galactose + GlcNAcbeta(1->2)Manalpha(1->3)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA 50% activity compared to Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA Bombyx mori ?
-
 ?
UDP-alpha-D-galactose + GlcNAcbeta(1->3)GalNAcalpha-pNP 32% activity compared to 4-nitrophenyl N-acetyl-beta-D-glucosaminide Bombyx mori ?
-
 ?
UDP-alpha-D-galactose + Manalpha(1->3)GlcNAcbeta(1->2)Manalpha(1->6)Manbeta(1->4)GlcNAc2-PA 100% activity Bombyx mori ?
-
 ?
UDP-alpha-D-galactose + N-acetyl-D-glucosamine
-
 Bombyx mori UDP + N-acetyllactosamine
-
 ?

Synonyms

Synonyms Comment Organism
beta-1,4-N-acetylgalactosaminyltransferase
-
 Bombyx mori
GalNAcT
-
 Bombyx mori

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Bombyx mori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Bombyx mori