Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.90 extracted from

  • Lau, K.; Thon, V.; Yu, H.; Ding, L.; Chen, Y.; Muthana, M.M.; Wong, D.; Huang, R.; Chen, X.
    Highly efficient chemoenzymatic synthesis of beta1-4-linked galactosides with promiscuous bacterial beta1-4-galactosyltransferases (2010), Chem. Commun. (Camb. ), 46, 6066-6068.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloned into pMAL-c4X vector and expressed in Escherichia coli Origami B (DE3) cells as an N-maltose binding protein (MBP)-tagged and C-His6-tagged fusion protein Helicobacter pylori
NmLgtB is cloned into pET15b vector and expressed in Escherichia coli BL21 (DE3) cells as N-terminal His6-tagged fusion protein. Under common expression conditions only a low amount of soluble active enzyme is obtained. The expression level is improved 15fold to 12.5 mg/L culture under osmotic stress condition using LB medium supplemented with 0.2% glucose and a higher concentration of NaCl Neisseria meningitidis

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Neisseria meningitidis
-
serogroup B strain
-

Storage Stability

Storage Stability Organism
-20°C, 10% glycerol, up to 14 months, no loss of activity Neisseria meningitidis
-20°C, 10% glycerol, up to 14 months, no loss of activity Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl-beta-D-GlcNAc + UDP-galactose Hp1–4GalT specifically recognizes GlcNAc-terminated glycans as acceptors Helicobacter pylori ?
-
?
additional information Hp1-4GalT can not use Glc, alpha-linked GlcNAc, and N-modified GlcNAc as acceptors but it can use 6-O-sulfated GlcNAc such as GlcNAc6S and GlcNAc6SbetaProN3 as suitable acceptor substrates Helicobacter pylori ?
-
?
additional information NmLgtB can use Glc, both alpha- and beta-linked GlcNAc, and N-modified but not 6-O-sulfated GlcNAc as acceptors Neisseria meningitidis ?
-
?
UDP-alpha-D-galactose + 4-methylumbelliferyl beta-D-glucopyranoside
-
Neisseria meningitidis UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-glucopyranoside
-
?
UDP-galactose + 4-methylumbelliferyl N-acetyl-beta-D-glucosamine NmLgtB can use both GlcNAc- and Glc-terminated glycans as acceptor substrates Neisseria meningitidis UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-N-acetyl-beta-D-glucosaminopyranoside
-
?

Synonyms

Synonyms Comment Organism
beta1-4-galactosyltransferase
-
Neisseria meningitidis
beta1-4-galactosyltransferase
-
Helicobacter pylori
beta1-4GalT
-
Neisseria meningitidis
beta1-4GalT
-
Helicobacter pylori
Hp1-4GalT
-
Helicobacter pylori
NmLgtB
-
Neisseria meningitidis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Neisseria meningitidis
37
-
assay at Helicobacter pylori

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Neisseria meningitidis
7.5
-
assay at Helicobacter pylori