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Literature summary for 2.4.1.90 extracted from

  • Boeggeman, E.; Ramakrishnan, B.; Kilgore, C.; Khidekel, N.; Hsieh-Wilson, L.C.; Simpson, J.T.; Qasba, P.K.
    Direct identification of nonreducing GlcNAc residues on N-glycans of glycoproteins using a novel chemoenzymatic method (2007), Bioconjug. Chem., 18, 806-814.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.90

Protein Variants

Protein Variants Comment Organism
Y289L/C342T site-directed mutagenesis, the mutant is able to transfer GalNAc from the sugar donor UDP-GalNAc to the acceptor, GlcNAc, with efficiency as good as that of galactose from UDP-Gal, in contrast to the wild-type enzyme, mutant substrate specificity with different donor substrate and oligosaccharides as acceptor substrates, mass spectrometry product analysis, overview, the C342T mutation does not alter enzyme activity, but increases the enzyme stability at room temperature Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetics, mutant enzyme, overview Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-galactose + N-acetylglucosamine Homo sapiens
-
 UDP + N-acetyllactosamine
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity of wild-type and mutant Y289L enzymes, overview Homo sapiens ?
-
 ?
UDP-galactose + N-acetylglucosamine
-
 Homo sapiens UDP + N-acetyllactosamine
-
 ?

Synonyms

Synonyms Comment Organism
beta4Gal-T1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Homo sapiens