Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | required for maximal activity at 0.75% w/v | Vigna radiata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | - |
Vigna radiata | |
Zn2+ | - |
Vigna radiata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
2-aminobenzaminated 1,4-linked beta-D-galactoheptaose | pH 6.5, 25°C | Vigna radiata | |
0.032 | - |
UDP-galactose | pH 6.5, 25°C | Vigna radiata |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Vigna radiata | 16020 | - |
microsome | - |
Vigna radiata | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | slight activation | Vigna radiata | |
Co2+ | activates, not as effective as Mn2+ | Vigna radiata | |
Mg2+ | activates, not as effective as Mn2+ | Vigna radiata | |
Mn2+ | required for maximal activity at 25 mM | Vigna radiata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + N-acetyl-D-glucosamine | Vigna radiata | - |
UDP + N-acetyllactosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vigna radiata | - |
at least 2 isoforms | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
hypocotyl | - |
Vigna radiata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with penta- and heptasaccharides generated from rhamnogalacturonan I, product determination by mass spectrometry, NMR, and enzymatic digestion analysis | Vigna radiata | ? | - |
? | |
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactoheptaose | fluorogenic synthetic acceptor substrate, elongation at the reducing end | Vigna radiata | UDP + 2-aminobenzaminated 1,4-linked beta-D-galactooctaose | - |
? | |
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactohexaose | fluorogenic synthetic acceptor substrate, elongation at the reducing end | Vigna radiata | UDP + 2-aminobenzaminated 1,4-linked beta-D-galactoheptaose | - |
? | |
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactooligosaccharides | fluorogenic synthetic acceptor substrates, enzyme transfers up to 8 galactose residues to the nonreducing ends of forming beta-1,4-linkages | Vigna radiata | UDP + ? | - |
? | |
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactopentaose | fluorogenic synthetic acceptor substrate, elongation at the reducing end | Vigna radiata | UDP + 2-aminobenzaminated 1,4-linked beta-D-galactohexaose | - |
? | |
UDP-galactose + 2-aminobenzaminated 1,4-linked beta-D-galactotetraose | fluorogenic synthetic acceptor substrate, elongation at the reducing end | Vigna radiata | UDP + 2-aminobenzaminated 1,4-linked beta-D-galactopentaose | - |
? | |
UDP-galactose + N-acetyl-D-glucosamine | - |
Vigna radiata | UDP + N-acetyllactosamine | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase | - |
Vigna radiata |
beta4Gal-T | - |
Vigna radiata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Vigna radiata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Vigna radiata |