Activating Compound | Comment | Organism | Structure |
---|---|---|---|
alpha-lactalbumin | stimulates transfer of galactose from UDPgalactose to N-acetylgalactosamine | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
enzymatically active soluble N-deglycosylated enzyme form | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km-value for ovalbumin for the N-deglycosylated recombinant enzyme is 6 mg/ml, and 15 mg/ml for th recombinant enzyme | Homo sapiens | |
0.091 | - |
UDPgalactose | recombinant enzyme | Homo sapiens | |
0.106 | - |
UDPgalactose | - |
Homo sapiens | |
0.143 | - |
UDPgalactose | recombinant N-deglycosylated enzyme | Homo sapiens | |
0.2 | - |
UDPgalactose | reaction with glucose, wild-type enzyme and N-deglycosylated recombinant enzyme | Homo sapiens | |
0.21 | - |
UDPgalactose | reaction with glucose, recombinant enzyme | Homo sapiens | |
1 | - |
D-glucose | recombinant enzyme | Homo sapiens | |
1.1 | - |
D-glucose | recombinant N-deglycosylated enzyme | Homo sapiens | |
2 | - |
D-glucose | - |
Homo sapiens | |
3.3 | - |
N-acetylglucosamine | - |
Homo sapiens | |
8.3 | - |
N-acetylglucosamine | recombinant N-deglycosylated enzyme | Homo sapiens | |
10 | - |
N-acetylglucosamine | recombinant enzyme | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
2 * 55000, SDS-PAGE | Homo sapiens |
106000 | - |
calculation from light-scattering experiments | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
milk | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6.9 | - |
N-deglycosylated recombinant enzyme | Homo sapiens |
8.4 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDPgalactose + glucose | in presence of alpha-lactalbumin | Homo sapiens | lactose + UDP | - |
? | |
UDPgalactose + N-acetylglucosamine | - |
Homo sapiens | UDP + N-acetyllactosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 55000, SDS-PAGE | Homo sapiens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
4 h, 18% loss of activity | Homo sapiens |
50 | - |
1 h, complete loss of activity | Homo sapiens |