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Literature summary for 2.4.1.9 extracted from
- Molecular and biochemical characteristics of the inulosucrase HugO from Streptomyces viridochromogenes DSM40736 (Tue494) (2017), Microbiology, 163, 1030-1041 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli NiCo21(DE3) cells | Streptomyces viridochromogenes |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not inhibited by EDTA | Streptomyces viridochromogenes |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 7.3 | - |
sucrose | at pH 6.0 and 45°C | Streptomyces viridochromogenes |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not dependent on Ca2+ | Streptomyces viridochromogenes |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + [(2->1)-beta-D-fructosyl]n | Streptomyces viridochromogenes | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | Streptomyces viridochromogenes DSM 40736 | - |
alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridochromogenes | - |
- |
- |
| Streptomyces viridochromogenes DSM 40736 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography | Streptomyces viridochromogenes |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sucrose + sucrose | - |
Streptomyces viridochromogenes | alpha-D-glucose + inulin-type fructan polymer | - |
? | |
| sucrose + sucrose | - |
Streptomyces viridochromogenes DSM 40736 | alpha-D-glucose + inulin-type fructan polymer | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Streptomyces viridochromogenes | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
| sucrose + [(2->1)-beta-D-fructosyl]n | - |
Streptomyces viridochromogenes DSM 40736 | alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 49000, calculated from amino acid sequence | Streptomyces viridochromogenes |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| fructansucrase | - |
Streptomyces viridochromogenes |
| FTF | - |
Streptomyces viridochromogenes |
| HugO | - |
Streptomyces viridochromogenes |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | the total activity increases until a plateau is reached from 50-55°C. Beyond 55°C the enzyme loses activity rapidly | Streptomyces viridochromogenes |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.5 | - |
sucrose | at pH 6.0 and 45°C | Streptomyces viridochromogenes | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | the optimal pH for the enzyme spans a relatively broad range, from pH 5.0 to 7.0. A relatively substantial amount of activity continues at the pH extremes of pH 4.0 (79.6 %) and pH 9.0 (49.2 %) | Streptomyces viridochromogenes |
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