Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.9 extracted from

  • Ni, D.; Zhu, Y.; Xu, W.; Bai, Y.; Zhang, T.; Mu, W.
    Biosynthesis of inulin from sucrose using inulosucrase from Lactobacillus gasseri DSM 20604 (2018), Int. J. Biol. Macromol., 109, 1209-1218 .
    View publication on PubMed
Search for more literature for 2.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Lactobacillus gasseri

Inhibitors

Inhibitors Comment Organism Structure
Al3+ about 95% residual activity at 1 mM Lactobacillus gasseri
Cu2+ about 55% residual activity at 1 mM Lactobacillus gasseri
Fe3+ about 65% residual activity at 1 mM Lactobacillus gasseri

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ about 105% activity at 1 mM Lactobacillus gasseri
Co2+ about 133% activity at 1 mM Lactobacillus gasseri
Fe2+ about 110% activity at 1 mM Lactobacillus gasseri
Mg2+ about 141% activity at 1 mM Lactobacillus gasseri
Mn2+ 157% activity at 1 mM Lactobacillus gasseri
Ni2+ about 140% activity at 1 mM Lactobacillus gasseri
Zn2+ about 138% activity at 1 mM Lactobacillus gasseri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 gel filtration Lactobacillus gasseri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus gasseri
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus gasseri DSM 20604
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Lactobacillus gasseri D3WYV9
-
-
Lactobacillus gasseri DSM 20604 D3WYV9
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-chelating Sepharose column chromatography Lactobacillus gasseri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Lactobacillus gasseri alpha-D-glucose + beta-D-fructose
-
 ?
sucrose + H2O
-
 Lactobacillus gasseri DSM 20604 alpha-D-glucose + beta-D-fructose
-
 ?
sucrose + sucrose
-
 Lactobacillus gasseri alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus gasseri DSM 20604 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus gasseri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus gasseri DSM 20604 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, SDS-PAGE Lactobacillus gasseri
monomer 1 * 62778, calculated from amino acid sequence Lactobacillus gasseri

Synonyms

Synonyms Comment Organism
sucrose:2,1-beta-D-fructan1-beta-D-fructosyltransferase
-
 Lactobacillus gasseri

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 optimal temperature for transfructosylation activity Lactobacillus gasseri
35
-
 optimal temperature for total activity Lactobacillus gasseri
45
-
 optimal temperature for sucrose hydrolysis activity Lactobacillus gasseri

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 60 the enzyme maintains 84% activity after incubation at 50°C for 3 h. 44% of the residual activity remains after 1 h at 55°C, and the enzyme loses 50% of its initial activity after incubation at 60°C for 20 min Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
 optimal pH for sucrose hydrolysis and transfructosylation activities Lactobacillus gasseri

pI Value

Organism Comment pI Value Maximum pI Value
Lactobacillus gasseri calculated from amino acid sequence
-
 5.2