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Literature summary for 2.4.1.9 extracted from

  • Ni, D.; Xu, W.; Zhu, Y.; Zhang, W.; Zhang, T.; Guang, C.; Mu, W.
    Inulin and its enzymatic production by inulosucrase Characteristics, structural features, molecular modifications and applications (2019), Biotechnol. Adv., 37, 306-318 .
    View publication on PubMed
Search for more literature for 2.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Bacillus subtilis Salipaludibacillus agaradhaerens
expressed in Escherichia coli Lactobacillus gasseri
expressed in Escherichia coli Leuconostoc citreum
expressed in Escherichia coli Lactobacillus johnsonii
expressed in Escherichia coli and Bacillus subtilis Limosilactobacillus reuteri
expressed in Escherichia coli and Bacillus subtilis Weissella confusa
expressed in Escherichia coli and Bacillus subtilis Bacillus sp. 217C-11

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Limosilactobacillus reuteri
Ca2+ activates Lactobacillus gasseri
Ca2+ activates Leuconostoc citreum
Ca2+ activates Lactobacillus johnsonii
additional information the enzyme is not affected by Ca2+ Salipaludibacillus agaradhaerens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose + [(2->1)-beta-D-fructosyl]n Streptococcus mutans
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Limosilactobacillus reuteri
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus gasseri
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Leuconostoc citreum
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus johnsonii
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Salipaludibacillus agaradhaerens
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Weissella confusa
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Streptomyces viridochromogenes
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Aspergillus sydowii
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Bacillus sp. 217C-11
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Weissella confusa MBFCNC-2(1)
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Streptococcus mutans JC-1
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Streptomyces viridochromogenes DSM 40736
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Streptococcus mutans JC-2
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Salipaludibacillus agaradhaerens WDG185
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus johnsonii NCC 533
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Leuconostoc citreum CW28
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Aspergillus sydowii IAM 2544
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Limosilactobacillus reuteri 121
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Limosilactobacillus reuteri TMW1.106
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus gasseri DSM 20604
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Lactobacillus gasseri DSM 20243
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n Streptococcus mutans BHT
-
 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus sydowii Q9P853
-
-
Aspergillus sydowii IAM 2544 Q9P853
-
-
Bacillus sp. 217C-11
-
-
-
Lactobacillus gasseri
-
-
-
Lactobacillus gasseri D3WYV9
-
-
Lactobacillus gasseri DSM 20243
-
-
-
Lactobacillus gasseri DSM 20604 D3WYV9
-
-
Lactobacillus johnsonii Q74K42
-
-
Lactobacillus johnsonii NCC 533 Q74K42
-
-
Leuconostoc citreum Q7X481
-
-
Leuconostoc citreum CW28 Q7X481
-
-
Limosilactobacillus reuteri
-
-
-
Limosilactobacillus reuteri Q0VJC5
-
-
Limosilactobacillus reuteri 121
-
-
-
Limosilactobacillus reuteri TMW1.106 Q0VJC5
-
-
Salipaludibacillus agaradhaerens A0A2D1CSM2
-
-
Salipaludibacillus agaradhaerens WDG185 A0A2D1CSM2
-
-
Streptococcus mutans
-
-
-
Streptococcus mutans BHT
-
-
-
Streptococcus mutans JC-1
-
-
-
Streptococcus mutans JC-2
-
-
-
Streptomyces viridochromogenes D9X8L9
-
-
Streptomyces viridochromogenes DSM 40736 D9X8L9
-
-
Weissella confusa D2WS87
-
-
Weissella confusa MBFCNC-2(1) D2WS87
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + sucrose
-
 Streptococcus mutans alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Limosilactobacillus reuteri alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus gasseri alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Leuconostoc citreum alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus johnsonii alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Salipaludibacillus agaradhaerens alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Weissella confusa alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Streptomyces viridochromogenes alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Aspergillus sydowii alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Bacillus sp. 217C-11 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Weissella confusa MBFCNC-2(1) alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Streptococcus mutans JC-1 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Streptomyces viridochromogenes DSM 40736 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Streptococcus mutans JC-2 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Salipaludibacillus agaradhaerens WDG185 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus johnsonii NCC 533 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Leuconostoc citreum CW28 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Aspergillus sydowii IAM 2544 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Limosilactobacillus reuteri 121 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Limosilactobacillus reuteri TMW1.106 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus gasseri DSM 20604 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Lactobacillus gasseri DSM 20243 alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + sucrose
-
 Streptococcus mutans BHT alpha-D-glucose + inulin-type fructan
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptococcus mutans alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Limosilactobacillus reuteri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus gasseri alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Leuconostoc citreum alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus johnsonii alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Salipaludibacillus agaradhaerens alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Weissella confusa alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptomyces viridochromogenes alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Aspergillus sydowii alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Bacillus sp. 217C-11 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Weissella confusa MBFCNC-2(1) alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptococcus mutans JC-1 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptomyces viridochromogenes DSM 40736 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptococcus mutans JC-2 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Salipaludibacillus agaradhaerens WDG185 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus johnsonii NCC 533 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Leuconostoc citreum CW28 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Aspergillus sydowii IAM 2544 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Limosilactobacillus reuteri 121 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Limosilactobacillus reuteri TMW1.106 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus gasseri DSM 20604 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Lactobacillus gasseri DSM 20243 alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?
sucrose + [(2->1)-beta-D-fructosyl]n
-
 Streptococcus mutans BHT alpha-D-glucose + [(2->1)-beta-D-fructosyl]n+1
-
 ?

Subunits

Subunits Comment Organism
? x * 45000, calculated from amino acid sequence Bacillus sp. 217C-11
? x * 63000, calculated from amino acid sequence Lactobacillus johnsonii
? x * 63000, calculated from amino acid sequence Lactobacillus gasseri
? x * 170000, calculated from amino acid sequence Leuconostoc citreum
? x * 47300, calculated from amino acid sequence Salipaludibacillus agaradhaerens
? x * 87000, calculated from amino acid sequence Limosilactobacillus reuteri

Synonyms

Synonyms Comment Organism
ISase
-
 Streptococcus mutans
ISase
-
 Limosilactobacillus reuteri
ISase
-
 Lactobacillus gasseri
ISase
-
 Leuconostoc citreum
ISase
-
 Lactobacillus johnsonii
ISase
-
 Salipaludibacillus agaradhaerens
ISase
-
 Weissella confusa
ISase
-
 Streptomyces viridochromogenes
ISase
-
 Aspergillus sydowii
ISase
-
 Bacillus sp. 217C-11

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
 Lactobacillus gasseri
45
-
-
 Leuconostoc citreum
45 50
-
 Bacillus sp. 217C-11
50
-
-
 Limosilactobacillus reuteri
50
-
-
 Lactobacillus gasseri
55
-
-
 Lactobacillus johnsonii
60
-
-
 Salipaludibacillus agaradhaerens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 50 the enzyme shows 50% activity after 420 min at 35°C and is inactive after 30 min at 50°C Leuconostoc citreum
50
-
 the enzyme shows 84% activity after 180 min at 50°C Lactobacillus gasseri

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5 5.5
-
 Lactobacillus gasseri
5 5.5
-
 Limosilactobacillus reuteri
5.5
-
-
 Lactobacillus gasseri
7 8
-
 Bacillus sp. 217C-11
7
-
-
 Lactobacillus johnsonii

pH Range

pH Minimum pH Maximum Comment Organism
6 10 the enzyme is active between pH 6.0 and 10.0 Salipaludibacillus agaradhaerens
6.5
-
-
 Leuconostoc citreum