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Literature summary for 2.4.1.9 extracted from

  • Rodriguez-Alegria, M.; Enciso-Rodriguez, A.; Ortiz-Soto, M.; Cassani, J.; Olvera, C.; Munguia, A.
    Fructooligosaccharide production by a truncated Leuconostoc citreum inulosucrase mutant (2010), Biocatal. Biotransform., 28, 51-59.
No PubMed abstract available
Search for more literature for 2.4.1.9

Cloned(Commentary)

Cloned (Comment) Organism
the truncated mutant IslA4 is expressed in Escherichia coli BL21(DE3) cells Leuconostoc citreum

Protein Variants

Protein Variants Comment Organism
L499F the mutation reduces the affinity for sucrose 3fold Leuconostoc citreum
additional information IslA4 is a truncated form of inulosucrase that contains only the catalytic domain, this truncated form is more hydrolytic than the wild type enzyme and produces both high-molecular-weight inulin and fructooligosaccharides Leuconostoc citreum
R618K mutant shows decreased activity compared to the wild type enzyme Leuconostoc citreum
R696K the mutant only produces inulin Leuconostoc citreum
S425A the mutant displays complex kinetic behavior in which the transfructosylation rate is described by first-order kinetics, while sucrose hydrolysis follows Michaelis-Menten behavior, the hydrolytic activity of S425A is reduced to about 52% of the converted sucrose Leuconostoc citreum
S425A the mutant no longer produces high-molecular-weight inulin Leuconostoc citreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
82.6
-
 sucrose wild type enzyme, at 30°C and pH 6.0 Leuconostoc citreum
125
-
 sucrose mutant enzyme S425A, at 30°C and pH 6.0 Leuconostoc citreum
126
-
 sucrose mutant enzyme R618K, at 30°C and pH 6.0 Leuconostoc citreum
308
-
 sucrose mutant enzyme L499F, at 30°C and pH 6.0 Leuconostoc citreum

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + (2,1-beta-D-fructosyl)n
-
 Leuconostoc citreum alpha-D-glucose + (2,1-beta-D-fructosyl)n+1
-
 ?

Synonyms

Synonyms Comment Organism
fructosyltransferase
-
 Leuconostoc citreum
FTF
-
 Leuconostoc citreum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 the wild type enzyme shows a half-life of 2.2 h at 35°C Leuconostoc citreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3
-
 sucrose mutant enzyme S425A, at 30°C and pH 6.0 Leuconostoc citreum
27
-
 sucrose mutant enzyme R618K, at 30°C and pH 6.0 Leuconostoc citreum
71
-
 sucrose wild type enzyme, at 30°C and pH 6.0 Leuconostoc citreum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.019
-
 sucrose mutant enzyme S425A, at 30°C and pH 6.0 Leuconostoc citreum
0.217
-
 sucrose mutant enzyme R618K, at 30°C and pH 6.0 Leuconostoc citreum
0.861
-
 sucrose wild type enzyme, at 30°C and pH 6.0 Leuconostoc citreum