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Literature summary for 2.4.1.9 extracted from

  • Del Moral, S.; Olvera, C.; Rodriguez, M.E.; Munguia, A.L.
    Functional role of the additional domains in inulosucrase (IslA) from Leuconostoc citreum CW28 (2008), BMC Biochem., 9, 6-16.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.9

Protein Variants

Protein Variants Comment Organism
additional information truncated version IslA2 of 102000 Da lacks C-terminal region, kinetic parameters and stability are similar to wild-type. Truncated version IslA3 of 80000 Da lacks both transition region and C-terminal region, protein is not attached to the cell surface such as wild-type. truncated version IslA4 of 64000 Da lacks part of the N-terminal region and both transition region and C-terminal region and show decrease in stability but increase in kcat value Leuconostoc citreum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 50 microM of EDTA and 5 min of incubation are enough to deplete truncated versions IslA4 and IslA3 of Ca2+ ions and activity, 1000 microM and 180 min are required to achieve the same effect with IslA2. Addition of Ca2+ restores activity Leuconostoc citreum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
24
-
 sucrose truncated version IslA2, pH 6.5, 30°C Leuconostoc citreum
38
-
 sucrose wild-type, pH 6.5, 30°C Leuconostoc citreum
142
-
 sucrose truncated version IslA4, pH 6.5, 30°C Leuconostoc citreum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ sequence contains a putative Ca2+ binding site. Addition of Ca2+ to EDTA-treated protein restores activity Leuconostoc citreum

Organism

Organism UniProt Comment Textmining
Leuconostoc citreum Q7X481 Enzyme is a multidomain fructosyltrasferase containing domains from glucosyltransferase
-
Leuconostoc citreum CW2 Q7X481 Enzyme is a multidomain fructosyltrasferase containing domains from glucosyltransferase
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sucrose + H2O
-
 Leuconostoc citreum alpha-D-glucose + beta-D-fructose
-
 ?
sucrose + H2O
-
 Leuconostoc citreum CW2 alpha-D-glucose + beta-D-fructose
-
 ?

Subunits

Subunits Comment Organism
More the C-terminal region of IslA is involved in anchoring the enzyme to the cell wall. In addition, besides conferring stability, the C-terminal domain modifies the accessibility to the active site, affecting its catalytic properties Leuconostoc citreum

Synonyms

Synonyms Comment Organism
IslA
-
 Leuconostoc citreum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 truncated versions IslA3, IslA4 Leuconostoc citreum
35
-
 both wild-type and truncated version IslA2 Leuconostoc citreum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35
-
 wild-type, half-life 420 min, truncated versions IslA2, half-life 407 min, IslA3, 346 min, IslA4, 128 min Leuconostoc citreum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25
-
 sucrose wild-type, pH 6.5, 30°C Leuconostoc citreum
28.2
-
 sucrose truncated version IslA2, pH 6.5, 30°C Leuconostoc citreum
105
-
 sucrose truncated version IslA4, pH 6.5, 30°C Leuconostoc citreum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 both wild-type and truncated versions IslA2, IslA3, IslA4 Leuconostoc citreum