Literature summary for 2.4.1.9 extracted from

Ozimek, L.K.; Kralj, S.; Kaper, T.; van der Maarel, M.J.; Dijkhuizen, L.
Single amino acid residue changes in subsite -1 of inulosucrase from Lactobacillus reuteri 121 strongly influence the size of products synthesized (2006), FEBS J., 273, 4104-4113.

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Protein Variants

Protein Variants	Comment	Organism
R423H	inactive mutant enzyme	Limosilactobacillus reuteri
R423K	altered fructooligosaccharide product pattern from sucrose, synthesizing a much lower amount of oligosaccharide and significantly more polymer than wild-type enzyme. KM-value for sucrose is 3.3fold higher than wild-type value. Vmax is 28.8fold lower than wild-type value	Limosilactobacillus reuteri
W271N	altered fructooligosaccharide product pattern from sucrose, synthesizing a much lower amount of oligosaccharide and significantly more polymer than wild-type enzyme. KM-value for sucrose is 15.6fold higher than wild-type value. Vmax is 16.9fold lower than wild-type value	Limosilactobacillus reuteri
W340N	inactive mutant enzyme	Limosilactobacillus reuteri

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
12	-	sucrose	37°C, wild-type enzyme	Limosilactobacillus reuteri
39	-	sucrose	37°C, mutant enzyme W271N	Limosilactobacillus reuteri
197	-	sucrose	37°C, mutant enzyme R423K	Limosilactobacillus reuteri

Organism

Organism	UniProt	Comment	Textmining
Limosilactobacillus reuteri	-	-	-
Limosilactobacillus reuteri 121	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3 sucrose + sucrose	enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]	Limosilactobacillus reuteri	3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1	-	?
3 sucrose + sucrose	enzyme produces a diverse range of fructooligosaccharide molecules and a minor amount of inulin polymer [with beta(21) linkages]	Limosilactobacillus reuteri 121	3 alpha-D-glucose + (2,1-beta-D-fructosyl)n+1	-	?

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Release 2023.1 (January 2023)