Cloned (Comment) | Organism |
---|---|
expression of Myc/His-tagged wild-type and mutant enzymes in Escherichia coli | Limosilactobacillus reuteri |
Protein Variants | Comment | Organism |
---|---|---|
D272N | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Limosilactobacillus reuteri |
D424N | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Limosilactobacillus reuteri |
E523Q | site-directed mutagenesis, highly reduced activity compared to the wild-type enzyme | Limosilactobacillus reuteri |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | Limosilactobacillus reuteri 121 | - |
alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Limosilactobacillus reuteri | - |
- |
- |
Limosilactobacillus reuteri 121 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Myc/His-tagged wild-type and mutant enzymes from Escherichia coli | Limosilactobacillus reuteri |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
sucrose + [(beta-D-Fruf-(2->1))n]-alpha-D-Glup = alpha-D-glucose + [(beta-D-Fruf-(2->1))n+1]-alpha-D-Glup | catalytic triad formed by essential residues D272, D424, and E523 | Limosilactobacillus reuteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00032 | - |
purified recombinant mutant D272N, inulin synthesizing activity | Limosilactobacillus reuteri |
0.00066 | - |
purified recombinant mutant D424N, inulin synthesizing activity | Limosilactobacillus reuteri |
0.0028 | - |
purified recombinant mutant E523Q, inulin synthesizing activity | Limosilactobacillus reuteri |
47 | - |
purified recombinant wild-type enzyme, inulin synthesizing activity | Limosilactobacillus reuteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + (2,1-beta-D-fructosyl)n | - |
Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | - |
? | |
sucrose + (2,1-beta-D-fructosyl)n | transferase activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + (2,1-beta-D-fructosyl)n+1 | i.e. inulin | ? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri | alpha-D-glucose + beta-D-fructose | - |
? | |
sucrose + H2O | hydrolytic activity | Limosilactobacillus reuteri 121 | alpha-D-glucose + beta-D-fructose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
fructosyltransferase | - |
Limosilactobacillus reuteri |
FTF | - |
Limosilactobacillus reuteri |
More | enzyme belongs to the family 68 of glucoside hydrolases | Limosilactobacillus reuteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Limosilactobacillus reuteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
assay at | Limosilactobacillus reuteri |