Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptococcus pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
D102A | inactive | Streptococcus pneumoniae |
D104A | inactive | Streptococcus pneumoniae |
D116A | the mutant remains active | Streptococcus pneumoniae |
D118A | the mutant remains active | Streptococcus pneumoniae |
D28A | the mutant remains active | Streptococcus pneumoniae |
D30A | the mutant remains active | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38799 | - |
x * 38799, calculated from amino acid sequence | Streptococcus pneumoniae |
39000 | - |
x * 39000, SDS-PAGE | Streptococcus pneumoniae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
serotype 6B | - |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity towards GlcNAcalpha-diphospho-O-(CH2)10CH3, UDP-D-glucose, Glcalpha-monophosphate, 4-nitrophenyl alpha-D-glucopyranoside, Glcalpha(1->2)Glc, Glcalpha-OMe, and Glcbeta-OMe | Streptococcus pneumoniae | ? | - |
? | |
UDP-alpha-D-galactose + Glcalpha-diphospho-O(CH2)10CH3 | wciN encodes an alpha-1,3-galactosyltransferase catalyzing the transfer of galactosyl from UDP-Gal onto the Glcalpha-diphosphate-lipid acceptor to form Galalpha(1->3)Glcalpha-diphosphate-lipid | Streptococcus pneumoniae | UDP + Galalpha(1->3)Glcalpha-diphospho-O(CH2)10CH3 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Streptococcus pneumoniae |
? | x * 38799, calculated from amino acid sequence | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,3-galactosyltransferase | - |
Streptococcus pneumoniae |
WciN | - |
Streptococcus pneumoniae |
General Information | Comment | Organism |
---|---|---|
physiological function | the wciN gene encodes a galactosyltransferase involved in the repeating unit assembly of Pneumococcus type 6B capsular polysaccharide | Streptococcus pneumoniae |