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Literature summary for 2.4.1.87 extracted from

  • Molina, P.; Knegtel, R.M.; Macher, B.A.
    Site-directed mutagenesis of glutamate 317 of bovine alpha -1,3Galactosyltransferase and its effect on enzyme activity: Implications for reaction mechanism (2007), Biochim. Biophys. Acta, 1770, 1266-1273.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli and COS-7 cells as a His-tagged fusion protein Bos taurus

Protein Variants

Protein Variants Comment Organism
E317A relative activity of mutant protein: 0.1% (compared to wild-type 100%) Bos taurus
E317C relative activity of mutant protein: 0.8% (compared to wild-type 100%) Bos taurus
E317D relative activity of mutant protein: 0.4% (compared to wild-type 100%) Bos taurus
E317H relative activity of mutant protein: 0.1% (compared to wild-type 100%) Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.31
-
 Gal-beta-1,4-GlcNAc-(CH2)8CO2CH3
-
 Bos taurus
0.67
-
 UDP-Gal
-
 Bos taurus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
 Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus P14769
-
-

Reaction

Reaction Comment Organism Reaction ID
UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-N-acetyl-D-glucosaminyl-R = UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-N-acetylglucosaminyl-R E317 is not the catalytic nucleophile proposed in the double-displacement mechanism. Either a different amino acid may act as the catalytic nucleophile or the reaction must proceed by a different mechanism Bos taurus
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 relative activity: 100% wild-type, 0.1% mutant E317A, 0.4% mutant E317D, 0.8% mutant E317C, 0.1% mutant E317H Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-Gal + Gal-beta-1,4-GlcNAc-(CH2)8CO2CH3
-
 Bos taurus UDP + ?
-
 ?

Synonyms

Synonyms Comment Organism
alpha-1,3galactosyltransferase
-
 Bos taurus
alpha1,3GalT
-
 Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Bos taurus