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Literature summary for 2.4.1.87 extracted from
- Defining the minimal size of catalytically active primate alpha 1,3 galactosyltransferase: structure-function studies on the recombinant truncated enzyme (1994), Glycobiology, 4, 193-201.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cloned from cDNA library, expression of full-length and truncated DNA in Escherichia coli and COS-1 cells, the truncated sequences are fused to protein A sequence resulting in expression of soluble chimeric proteins, DNA sequence determination and analysis | Callithrix jacchus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | truncation of 3 amino acid residues from the C-terminus, and a frame shift mutation result in a complete loss of activity | Callithrix jacchus |
| N375V | exchange and truncation of terminal V376, 70% loss of activity | Callithrix jacchus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
amino acid sequence alignment | Callithrix jacchus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Callithrix jacchus | - |
marmoset monkey | - |
| no activity in african green monkey | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| B95-8 cell | - |
Callithrix jacchus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
Callithrix jacchus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? |  |
| UDP-galactose + N-acetyllactosamine | i.e. beta-D-galactosyl-1,4-N-acetyl-D-glucosamine | Callithrix jacchus | UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine | - |
ir | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| alpha-1,3-galactosyltransferase | - |
Callithrix jacchus |
| alpha1,3GT | - |
Callithrix jacchus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Callithrix jacchus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Callithrix jacchus |
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Release 2023.1 (January 2023)
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