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Literature summary for 2.4.1.87 extracted from

  • Van den Eijnden, D.H.; Blanken, W.M.; Winterwerp, H.; Schiphorst, W.E.C.M.
    Identification and characterization of an UDP-Gal: N-acetyllactosaminide alpha-1,3-D-galactosyltransferase in calf thymus (1983), Eur. J. Biochem., 134, 523-530.
    View publication on PubMed
Search for more literature for 2.4.1.87

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 activates Bos taurus
Triton X-100 at 0.8% v/v 4fold stimulation Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
EDTA weak Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 UDP-galactose
-
 Bos taurus
2.7
-
 N-acetyllactosamine
-
 Bos taurus
3.7
-
 asialo-alpha1-acid glycoprotein
-
 Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane bound Bos taurus 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ slight activation Bos taurus
Mn2+ Km 6.1 mM Bos taurus
Mn2+ pronounced requirement Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus functions in the biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins ?
-
 ?
additional information Bos taurus functions in biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information functions in the biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins Bos taurus ?
-
 ?
additional information functions in biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins Bos taurus ?
-
 ?
UDP-galactose + asialo-alpha1-acid glycoprotein
-
 Bos taurus UDP + alpha-D-galactosyl-asialo-alpha1-acid glycoprotein
-
 ?
UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme introduces galactosyl residues in alpha anomeric configuration to the Galbeta(1-4)GlcNAc units on the acceptor substrate Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-galactose + glycoprotein the nonreducing terminal N-acetyllactosamine residue of glycoproteins can act as acceptor Bos taurus UDP + alpha-D-galactosylglycoprotein
-
 ?
UDP-galactose + lactose
-
 Bos taurus UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose
-
 ?
UDP-galactose + N-acetyllactosamine i.e. beta-D-galactosyl-1,4-N-acetyl-D-glucosamine Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5 7 cacodylate buffer Bos taurus
6
-
 acetate buffer Bos taurus