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Literature summary for 2.4.1.86 extracted from

  • Basu, M.; Weng, S.A.; Tang, H.; Khan, F.; Rossi, F.; Basu, S.
    Biosynthesis in vitro of neolactotetraosylceramide by a galactosyltransferase from mouse T-lymphoma: purification and kinetic studies; synthesis of neolacto and polylactosamine core (1996), Glycoconjugate J., 13, 423-432.
    View publication on PubMed
Search for more literature for 2.4.1.86

General Stability

General Stability Organism
the purified enzyme is partially stabilized in presence of phospholipid liposomes Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
 N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 Mus musculus
0.25
-
 UDPgalactose
-
 Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ required Mus musculus

Organism

Organism UniProt Comment Textmining
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
P-1798 cell mineral oil induced Mus musculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.344
-
-
 Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDPgalactose + N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 Mus musculus UDP + 1,4-beta-D-galactosyl-N-acetyl-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-D-glucosylceramide
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
 Mus musculus