Protein Variants | Comment | Organism |
---|---|---|
I253N | higher value for the apparent Km-value for dolichyl phosphate when assayed in detergent solution, mutation has no effect on Km-value when the enzyme is reconstituted with phosphatidylethanolamine | Saccharomyces cerevisiae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0015 | - |
GDPmannose | wild-type enzyme assayed in solution of Nonidet P-40 | Saccharomyces cerevisiae | |
0.0025 | - |
dolichyl phosphate | wild-type enzyme reconstituted with phosphatidylethanolamine | Saccharomyces cerevisiae | |
0.0027 | - |
dolichyl phosphate | wild-type enzyme reconstituted with phosphatidylethanolamine | Saccharomyces cerevisiae | |
0.0106 | - |
dolichyl phosphate | wild-type enzyme assayed in solution of Nonidet P-40 | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | - |
recombinant enzyme | - |
Purification (Comment) | Organism |
---|---|
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDPmannose + dolichyl phosphate | - |
Saccharomyces cerevisiae | GDP + dolichyl D-mannosyl phosphate | - |
? |