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Literature summary for 2.4.1.81 extracted from

  • Cho, K.; Kim, T.; Le, T.; Nguyen, H.; Oh, S.; Pandey, R.; Sohng, J.
    Altering UDP-glucose donor substrate specificity of Bacillus licheniformis glycosyltransferase towards TDP-glucose (2019), J. Microbiol. Biotechnol., 29, 268-273 .
    View publication on PubMed
Search for more literature for 2.4.1.81

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus licheniformis

Crystallization (Commentary)

Crystallization (Comment) Organism
molecular modeling and docking of substrates TDP-glucose and 7-hydroxyflavone Bacillus licheniformis

Protein Variants

Protein Variants Comment Organism
H14A 81% of wild-type activity with UDP-glucose, 9% of wild-type activity with TDP-glucose Bacillus licheniformis
R282W increase in activity with TDP-glucose Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.07
-
 TDP-glucose wild-type, pH 8.0, temperature not specified in the publication Bacillus licheniformis
2.19
-
 TDP-glucose mutant R282W, pH 8.0, temperature not specified in the publication Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimum concentration in the assay is 10 mM Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis Q65JC2
-
-
Bacillus licheniformis DSM 13 Q65JC2
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + 7,8-dihydroxyflavone
-
 Bacillus licheniformis ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
-
 ?
ADP-glucose + 7,8-dihydroxyflavone
-
 Bacillus licheniformis DSM 13 ADP + 7-O-beta-D-glucosyl-7,8-dihydroxyflavone
-
 ?
CDP-glucose + 4',7-dihydroxyflavone
-
 Bacillus licheniformis CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
-
 ?
CDP-glucose + 4',7-dihydroxyflavone
-
 Bacillus licheniformis DSM 13 CDP + 7-O-beta-D-glucosyl-4',7-dihydroxyflavone
-
 ?
additional information UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used Bacillus licheniformis ?
-
-
additional information UDP is preferred over other NDP-sugars. The conversion of 7-hydroxyflavone to its glucoside is 33.6, 19.4, 22.1 and 15% when TDP-, CDP-, ADP- and GDP-glucose are used Bacillus licheniformis DSM 13 ?
-
-
TDP-glucose + curcumin
-
 Bacillus licheniformis TDP + 7-O-beta-D-glucosyl-curcumin
-
 ?
TDP-glucose + curcumin
-
 Bacillus licheniformis DSM 13 TDP + 7-O-beta-D-glucosyl-curcumin
-
 ?
UDP-glucose + 7-hydroxyflavone
-
 Bacillus licheniformis UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
-
 ?
UDP-glucose + 7-hydroxyflavone
-
 Bacillus licheniformis DSM 13 UDP + 7-O-beta-D-glucosyl-7-hydroxyflavone
-
 ?

Subunits

Subunits Comment Organism
? x * 46000, SDS-PAGE Bacillus licheniformis

Synonyms

Synonyms Comment Organism
YjiC
-
 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Bacillus licheniformis