Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CHAPS | i.e. 3-[(3-cholamidopropyl)dimethylamonio]-1propanesulfonate | Sus scrofa | |
detergent | CHAPS stimulates | Sus scrofa | |
NADH | omission in assay containing Golgi vesicles leads to loss of about 90% activity | Sus scrofa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Sus scrofa | |
Triton X-100 | - |
Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
UDP-glucose | - |
Sus scrofa | |
0.054 | - |
ceramide | - |
Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Sus scrofa | 5794 | - |
Golgi membrane | - |
Sus scrofa | 139 | - |
membrane | - |
Sus scrofa | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | stimulates | Sus scrofa | |
Mn2+ | stimulates | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | Sus scrofa | catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis | UDP + D-glucosyl-N-acylsphingosine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
submaxillary ganglion | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0000065 | - |
- |
Sus scrofa |
0.0007 | - |
Golgi fraction | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + N-acylsphingosine | i.e. ceramide | Sus scrofa | UDP + D-glucosyl-N-acylsphingosine | i.e. glucosylceramide | ? | |
UDP-glucose + N-acylsphingosine | catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis | Sus scrofa | UDP + D-glucosyl-N-acylsphingosine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
sharp fall of activity below and above pH 6.5 | Sus scrofa |
6.5 | - |
- |
Sus scrofa |